CD25 / PerCP-eFluor 710 / PC61.5
CD25 / PerCP-eFluor 710 / PC61.5
Product Details
| Supplier | Thermo Fisher Scientific | |
|---|---|---|
| Catalog #: | 46-0251-80 (View supplier product page) | |
| Size | 25 μg | |
| Price | $111.00 | |
| Antigen | CD25 | |
| Clone | PC61.5 | |
| Host | Rat | |
| Isotype | IgG1 λ | |
| Conjugate | PerCP-eFluor 710 | |
| Target Species | Mouse | |
| Applications | FC | |
| Description | CD25 Monoclonal Antibody (PC61.5), PerCP-eFluor 710, eBioscience™ |
About CD25 and PerCP-eFluor 710
| CD25 | The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency. Patients with severe Coronavirus Disease 2019 (COVID-19), the disease caused by the novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), have significantly elevated levels of IL2R in their plasma. Similarly, serum IL-2R levels are found to be elevated in patients with different types of carcinomas. Certain IL2RA and IL2RB gene polymorphisms have been associated with lung cancer risk. [provided by RefSeq, Jul 2020] | |
|---|---|---|
| PerCP-eFluor 710 | PerCP-eFluor™ 710 (PerCP-eF710) is a red-emitting tandem fluorophore that combines PerCP and eF710. The donor molecule, PerCP can be excited by the 488-nm blue laser and and transfers energy to the acceptor molecule, eF710, which emitts light that can be captured with a 695/40 nm bandpass filter. PerCP-eF710 has an excitation peak at 482 nm and an emission peak at 710 nm, and is considered a slightly-red shifted but brighter alternative to PerCP-Cy5.5. This dye and associated products are mostly used for flow cytometry. This dye is part of the larger eFluor™ dye family, developed by eBioscience which is now part of ThermoFisher Scientific. |
Images
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BALB/c mouse splenocytes were stained with CD4 Monoclonal Antibody, FITC (Product # 11-0041-82) and 0.125 µg of Rat IgG1 kappa Isotype Control, PerCP-eFluor 710 (Product # 46-4301-80) (left) or 0.125 µg of CD25 Monoclonal Antibody, PerCP-eFluor 710 (right). Cells in the lymphocyte gate were used for analysis. |
Citations
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Reviews & Ratings
PerCP-eFluor 710 Excitation and Emission Spectra
$111.00
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