CD25 Monoclonal / Alexa Fluor 647 / PC61.5
CD25 Monoclonal / Alexa Fluor 647 / PC61.5
Product Details
| Supplier | Thermo Fisher Scientific | |
|---|---|---|
| Catalog #: | 51-0251-82 (View supplier product page) | |
| Size | 100 μg | |
| Price | $241.00 | |
| Antigen | CD25 | |
| Clone | PC61.5 | |
| Host | Rat | |
| Isotype | IgG1 λ | |
| Conjugate | Alexa Fluor 647 | |
| Target Species | Mouse | |
| Applications | FC | |
| Description | CD25 Monoclonal Antibody (PC61.5), Alexa Fluor 647, eBioscience |
About CD25 and Alexa Fluor 647
| CD25 | The interleukin 2 (IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency. Patients with severe Coronavirus Disease 2019 (COVID-19), the disease caused by the novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), have significantly elevated levels of IL2R in their plasma. Similarly, serum IL-2R levels are found to be elevated in patients with different types of carcinomas. Certain IL2RA and IL2RB gene polymorphisms have been associated with lung cancer risk. [provided by RefSeq, Jul 2020] | |
|---|---|---|
| Alexa Fluor 647 | Alexa Fluor™ 647 (AF647, Alexa 647) has an excitation peak at 650 nm and an emission peak at 665 nm, and is spectrally similar to Cy®5 (GE Healthcare), iFluor® 647 (ATT Bioquest), and DyLight™ 650 (Thermo Fisher Scientific). Alexa 647 is commonly used for flow cytometry, microscopy, super-resolution microscopy applications. It is very bright, photostable, and pH insensitive, all of which contribute to sensitive detection while using this dye. |
Images
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Swiss Webster mouse splenocytes were stained with CD4 Monoclonal Antibody, FITC (Product # 11-0042-82) and 0.125 µg of Rat IgG1 kappa Isotype Control, Alexa Fluor 647 (left) or 0.125 µg of CD25 Monoclonal Antibody, Alexa Fluor 647 (right). Cells in the lymphocyte gate were used for analysis. |
Citations
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Reviews & Ratings
Alexa Fluor 647 Excitation and Emission Spectra
$241.00
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