Arginase 1 Monoclonal / PerCP-eFluor 710 / A1exF5
Arginase 1 Monoclonal / PerCP-eFluor 710 / A1exF5
Citations:
(9)
Product Details
| Supplier | Thermo Fisher Scientific | |
|---|---|---|
| Catalog #: | 46-3697-80 (View supplier product page) | |
| Size | 25 μg | |
| Price | $171.00 | |
| Antigen | Arginase 1 | |
| Clone | A1exF5 | |
| Host | Rat | |
| Isotype | IgG2a κ | |
| Conjugate | PerCP-eFluor 710 | |
| Target Species | Human, Mouse | |
| Applications | FC | |
| Description | Arginase 1 Monoclonal Antibody (A1exF5), PerCP-eFluor 710, eBioscience |
About Arginase 1 and PerCP-eFluor 710
| Arginase 1 | Arginase catalyzes the hydrolysis of arginine to ornithine and urea. At least two isoforms of mammalian arginase exist (types I and II) which differ in their tissue distribution, subcellular localization, immunologic crossreactivity and physiologic function. The type I isoform encoded by this gene, is a cytosolic enzyme and expressed predominantly in the liver as a component of the urea cycle. Inherited deficiency of this enzyme results in argininemia, an autosomal recessive disorder characterized by hyperammonemia. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011] | |
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| PerCP-eFluor 710 | PerCP-eFluor™ 710 (PerCP-eF710) is a red-emitting tandem fluorophore that combines PerCP and eF710. The donor molecule, PerCP can be excited by the 488-nm blue laser and and transfers energy to the acceptor molecule, eF710, which emitts light that can be captured with a 695/40 nm bandpass filter. PerCP-eF710 has an excitation peak at 482 nm and an emission peak at 710 nm, and is considered a slightly-red shifted but brighter alternative to PerCP-Cy5.5. This dye and associated products are mostly used for flow cytometry. This dye is part of the larger eFluor™ dye family, developed by eBioscience which is now part of ThermoFisher Scientific. |
Images
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C57BL/6 mouse bone marrow derived macrophages were polarized for 24 hours with either LPS (Product # 00-4976-93) and IFN-gamma (Product # 16-7311-85) to make M1 macrophages (left) or with IL-4 (Product # BMS338) to make M2a macrophages (right). Cells were surface stained with F4/80 Monoclonal Antibody, FITC (Product # 11-4801-82) then stained intracellularly, using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol, with 0.125 µg Arginase 1 Monoclonal Antibody, PerCP-eFluor 710. Total viable cells were used for analysis, as determined by Fixable Viability Dye eFluor 450 (Product # 65-0863-18). |
Citations
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Reviews & Ratings
PerCP-eFluor 710 Excitation and Emission Spectra
$171.00
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