Arginase 1 Monoclonal / Alexa Fluor 700 / A1exF5

Citations: (1)
Product Details
Supplier Thermo Fisher Scientific
Catalog #: 56-3697-82 (View supplier product page)
Size 100 μg
Price $357.00
Antigen Arginase 1
Clone A1exF5
Host Rat
Isotype IgG2a κ
Conjugate Alexa Fluor 700
Target Species Human, Mouse
Applications FC
Description Arginase 1 Monoclonal Antibody (A1exF5), Alexa Fluor 700, eBioscience
About Arginase 1 and Alexa Fluor 700
Arginase 1 Arginase catalyzes the hydrolysis of arginine to ornithine and urea. At least two isoforms of mammalian arginase exist (types I and II) which differ in their tissue distribution, subcellular localization, immunologic crossreactivity and physiologic function. The type I isoform encoded by this gene, is a cytosolic enzyme and expressed predominantly in the liver as a component of the urea cycle. Inherited deficiency of this enzyme results in argininemia, an autosomal recessive disorder characterized by hyperammonemia. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]
Alexa Fluor 700 Alexa Fluor™ 700 (AF700, Alexa 700) has an excitation peak at 702 nm and an emission peak at 723 nm, and is spectrally similar to iFluor® 680 (ATT Bioquest) and iFluor® 700 (ATT Bioquest). Alexa 700 is commonly used in Flow Cytometry and is similar in size, brightness and application to iFluor® 700 and Vio® R720.
Images
  Arginase 1 Alexa Fluor 700 Thermo Fisher Scientific antibody anti target:Human, Mouse host:Rat isotype:IgG2a κ clone:A1exF5 C57BL/6 mouse bone marrow derived macrophages were polarized for 24 hours with either LPS (Produc...

C57BL/6 mouse bone marrow derived macrophages were polarized for 24 hours with either LPS (Product # 00-4976-93) and IFN-gamma (Product # 16-7311-85) to make M1 macrophages (left) or with IL-4 (Product # BMS338) to make M2a macrophages (right). Cells were surface stained with F4/80 Monoclonal Antibody, FITC (Product # 11-4801-82) then stained intracellularly, using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol, with 0.06 µg Arginase 1 Monoclonal Antibody, Alexa Fluor 700. Total viable cells were used for analysis, as determined by Fixable Viability Dye eFluor 450 (Product # 65-0863-18).

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Alexa Fluor 700 Excitation and Emission Spectra