Flow cytometry of Phospho-CHK1 in HeLa cells. Sample was incubated with Phospho-CHK1 monoclonal antibody (Product # MA5-27987) at a dilution of 0.01 µg/mL. Cells were treated with secondary antibody only negative control (light blue), untreated (red), treated with calyculin A (green), untreated and blocked with phospho-peptide (black), CalA and blocked with phospho peptide (gold), untreated and blocked with non-phospho peptide (dark blue), and CalA and blocked with non-phospho peptide (purple).

Flow cytometry of Phospho-CHK1 in NIH3T3 cells. Sample was incubated with Phospho-CHK1 monoclonal antibody (Product # MA5-27987) at a dilution of 0.1 µg/mL. Cells were treated with secondary antibody only (blue), isotype control (orange) or primary antibody (green).

Flow cytometry of Phospho-CHK1 in HeLa cells. Sample was incubated with Phospho-CHK1 monoclonal antibody (Product # MA5-27987) at a dilution of 1 µg/mL. Cells were treated with secondary antibody only negative control (blue), untreated (red) or treated with UV plus TPA (green).

Flow cytometry of Phospho-CHK1 in HeLa cells. Sample was incubated with Phospho-CHK1 monoclonal antibody (Product # MA5-27987) at a dilution of 0.01 µg/mL. Cells were treated with negative control (blue), treated with imatinib (red) and treated with pervanadate (green).