Flow cytometry of Phospho-BAD in NIH3T3 cells. Sample was incubated with Phospho-BAD monoclonal antibody (Product # MA5-28043) at a dilution of 0.05 µg/mL. Cells were unstained and treated with imatinib as negative control (blue), stained and treated with imatinib (red) or treated with pervanadate (green).

Flow cytometry of Phospho-BAD in U937 cells. Sample was incubated with Phospho-BAD monoclonal antibody (Product # MA5-28043) at a dilution of 0.1 µg/mL. Cells were treated with secondary antibody only negative control (blue), Calyculin A-treated (green), CalA and blocked with phospho peptide (gold), and CalA and blocked with non-phospho peptide (purple).

Flow cytometry of Phospho-BAD in NIH3T3 cells. Sample was incubated with Phospho-BAD monoclonal antibody (Product # MA5-28043) at a dilution of 0.1 µg/mL. Cells were treated with secondary antibody only (blue), isotype control (orange) or primary antibody (green).