CD22 / FITC / OTI1F12

Product Details
Supplier Novus Biologicals, a Bio-Techne brand
Catalog #: NBP2-74186F (View supplier product page)
Size 0.1 ml
Price $679.00
Antigen CD22
Clone OTI1F12
Host Mouse
Isotype IgG1
Conjugate FITC
Target Species Canine, Human, Monkey, Mouse, Rat
Applications ICC, IF
Description Mouse Siglec-2/CD22 antibody [FITC] detects Human, Mouse, Rat, Canine, Monkey
About CD22 and FITC
CD22 Predicted to enable CD4 receptor binding activity; protein phosphatase binding activity; and sialic acid binding activity. Involved in B cell activation; negative regulation of B cell receptor signaling pathway; and regulation of endocytosis. Located in early endosome and recycling endosome. [provided by Alliance of Genome Resources, Apr 2022]
FITC Fluorescein isothiocyanate (FITC) has an excitation peak at 495 nm and an emission peak at 519 nm. The name FITC is a misnomer in that the isothiocyanate is a reactive form of this dye. Once FITC is conjugated to an antibody, it is simply Fluorescein conjugated. FITC is one of the most widely used dyes for fluorescent applications, therefore most instruments come standard with a 488 nm laser and FITC filter set up. FITC is commonly conjugated to secondary antibodies and used in applications such as flow cytometry, immunocytochemistry, and immunohistochemistry. FITC is relatively dim, sensitive to photobleaching and it is susceptible to changes is pH. There are better performing alternatives to FITC, like Vio®Bright 515, Alexa Fluor™ 488, iFluor® 488, CF®488A and DY-488. FITC is a long-time generic dye with no sole manufacturer or trademark.
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FITC Excitation and Emission Spectra