MDM4 / Alexa Fluor 647 / OTI4G5

Product Details
Supplier Novus Biologicals, a Bio-Techne brand
Catalog #: NBP2-72605AF647 (View supplier product page)
Size 0.1 ml
Price $679.00
Antigen MDM4
Clone OTI4G5
Host Mouse
Isotype IgG2b
Conjugate Alexa Fluor 647
Target Species Human
Applications WB, IHC, ICC/IF
Description Mouse MDMX antibody [Alexa Fluor 647] detects Human
About MDM4 and Alexa Fluor 647
MDM4 This gene encodes a nuclear protein that contains a p53 binding domain at the N-terminus and a RING finger domain at the C-terminus, and shows structural similarity to p53-binding protein MDM2. Both proteins bind the p53 tumor suppressor protein and inhibit its activity, and have been shown to be overexpressed in a variety of human cancers. However, unlike MDM2 which degrades p53, this protein inhibits p53 by binding its transcriptional activation domain. This protein also interacts with MDM2 protein via the RING finger domain, and inhibits the latter's degradation. So this protein can reverse MDM2-targeted degradation of p53, while maintaining suppression of p53 transactivation and apoptotic functions. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Feb 2011]
Alexa Fluor 647 Alexa Fluor™ 647 (AF647, Alexa 647) has an excitation peak at 650 nm and an emission peak at 665 nm, and is spectrally similar to Cy®5 (GE Healthcare), iFluor® 647 (ATT Bioquest), and DyLight™ 650 (Thermo Fisher Scientific). Alexa 647 is commonly used for flow cytometry, microscopy, super-resolution microscopy applications. It is very bright, photostable, and pH insensitive, all of which contribute to sensitive detection while using this dye.
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Alexa Fluor 647 Excitation and Emission Spectra