FluoroFinder

	Supplier	Miltenyi Biotec
	Catalog #:	130-117-668 (View supplier product page)
	Size	150 µg in 1 mL
	Price	$195.00
	Antigen	IFN-gamma
	Clone	REA638
	Host	Human
	Isotype	IgG1
	Conjugate	FITC
	Target Species	Mouse
	Applications	FC
	Description	Clone REA638 recognizes the mouse interferon-γ (IFN-γ) antigen, a secreted protein which is produced by activated T, B, and NK cells. IFN-γ is a pleiotropic cytokine responsible for macrophage activation and differentiation. It induces transcription of several proinflammatory genes, such as inducible NO synthase, cyclooxygenase-2, and IL-1β, as well as MHC proteins. IFN-γ, in synergy with other cytokines such as TNF-α, inhibits proliferation of normal and transformed cells. Immunomodulatory effects of IFN-γ are exerted on a wide range of cell types expressing the high affinity receptors for IFN-γ. \| Additional information: Clone REA638 displays negligible binding to Fc receptors.

	IFN-gamma	This gene encodes a soluble cytokine that is a member of the type II interferon class. The encoded protein is secreted by cells of both the innate and adaptive immune systems. The active protein is a homodimer that binds to the interferon gamma receptor which triggers a cellular response to viral and microbial infections. Mutations in this gene are associated with an increased susceptibility to viral, bacterial and parasitic infections and to several autoimmune diseases. [provided by RefSeq, Dec 2015]
	FITC	Fluorescein isothiocyanate (FITC) has an excitation peak at 495 nm and an emission peak at 519 nm. The name FITC is a misnomer in that the isothiocyanate is a reactive form of this dye. Once FITC is conjugated to an antibody, it is simply Fluorescein conjugated. FITC is one of the most widely used dyes for fluorescent applications, therefore most instruments come standard with a 488 nm laser and FITC filter set up. FITC is commonly conjugated to secondary antibodies and used in applications such as flow cytometry, immunocytochemistry, and immunohistochemistry. FITC is relatively dim, sensitive to photobleaching and it is susceptible to changes is pH. There are better performing alternatives to FITC, like Vio®Bright 515, Alexa Fluor™ 488, iFluor® 488, CF®488A and DY-488. FITC is a long-time generic dye with no sole manufacturer or trademark.

$195.00
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IFN-gamma / FITC / REA638