CD35 / PE / REA1133

Citations: (2)
Product Details
Supplier Miltenyi Biotec
Catalog #: 130-119-625 (View supplier product page)
Size 30 tests in 60 µL
Price $125.00
Antigen CD35
Clone REA1133
Host Human
Isotype IgG1
Conjugate PE
Target Species Human
Applications FC, MICS (MACSima Imaging Cyclic Staining), IF, IHC
Description Clone REA1133 recognizes the human cell surface protein CD35, the C3b/C4b receptor, also known as CR1 or complement receptor type I. Four CD35 allotypes with different molecular weights are known; 190, 220, 250, and 280 kDa. CD35 is expressed on hematopoietic cells such as granulocytes, monocytes, B cells, subsets of NK and T cells, erythrocytes, and follicular dendritic cells (DCs). Known functions of CD35 include transport of C3 complexes on erythrocytes, complement activation, phagocytosis, and inhibition of the activation-induced proliferation of T and B cells. | Additional information: Clone REA1133 displays negligible binding to Fc receptors.
About CD35 and PE
CD35 This gene is a member of the receptors of complement activation (RCA) family and is located in the 'cluster RCA' region of chromosome 1. The genome is polymorphic at this locus with allele-specific splice variants encoding different isoforms, based on the presence/absence of long homologous repeats (LHRs). The gene encodes a monomeric single-pass type I membrane glycoprotein found on erythrocytes, leukocytes, glomerular podocytes, and splenic follicular dendritic cells. The Knops blood group system is a system of antigens located on this protein. The protein mediates cellular binding to particles and immune complexes that have activated complement. Decreases in expression of this protein and/or mutations in this gene have been associated with gallbladder carcinomas, mesangiocapillary glomerulonephritis, systemic lupus erythematosus, sarcoidosis and Alzheimer's disease. Mutations in this gene have also been associated with a reduction in Plasmodium falciparum rosetting, conferring protection against severe malaria. [provided by RefSeq, May 2020]
PE Phycoerythrin (PE, R-PE) is a red-emitting fluorescent protein-chromophore complex that can be excited the 488-nm blue, 532-nm green, or 561-nm yellow-green laser with increasing efficiency and captured with a 586/14 nm bandpass filter. PE has an excitation peak at 565 nm and an emission peak at 578 nm. PE is 240kD in size and has an extinction coefficient of ~2x10^6 which makes it one of the brightest fluorophores available and a potent donor upon which to build tandem fluorophores with longer Stoke's Shifts.
Citations
PMID 2411809
PMID 2972794
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PE Excitation and Emission Spectra