FluoroFinder

	Supplier	Miltenyi Biotec
	Catalog #:	130-117-775 (View supplier product page)
	Size	30 µg in 200 µL
	Price	$55.00
	Antigen	CD34
	Clone	REA383
	Host	Human
	Isotype	IgG1
	Conjugate	FITC
	Target Species	Mouse
	Applications	FC
	Description	Clone REA383 recognizes the mouse CD34 antigen, a highly glycosylated single-pass type I membrane protein also known as mucosialin. CD34 is highly expressed in hematopoietic progenitor cell lines, as well as on endothelial cells, brain, and testis. It is a adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. CD34 acts as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. \| Additional information: Clone REA383 displays negligible binding to Fc receptors.

	CD34	The protein encoded by this gene may play a role in the attachment of stem cells to the bone marrow extracellular matrix or to stromal cells. This single-pass membrane protein is highly glycosylated and phosphorylated by protein kinase C. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]
	FITC	Fluorescein isothiocyanate (FITC) has an excitation peak at 495 nm and an emission peak at 519 nm. The name FITC is a misnomer in that the isothiocyanate is a reactive form of this dye. Once FITC is conjugated to an antibody, it is simply Fluorescein conjugated. FITC is one of the most widely used dyes for fluorescent applications, therefore most instruments come standard with a 488 nm laser and FITC filter set up. FITC is commonly conjugated to secondary antibodies and used in applications such as flow cytometry, immunocytochemistry, and immunohistochemistry. FITC is relatively dim, sensitive to photobleaching and it is susceptible to changes is pH. There are better performing alternatives to FITC, like Vio®Bright 515, Alexa Fluor™ 488, iFluor® 488, CF®488A and DY-488. FITC is a long-time generic dye with no sole manufacturer or trademark.

$55.00
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CD34 / FITC / REA383