CD235a / APC / REA175

Citations: (2)
Product Details
Supplier Miltenyi Biotec
Catalog #: 130-118-356 (View supplier product page)
Size 100 tests in 200 µL
Price $335.00
Antigen CD235a
Clone REA175
Host Human
Isotype IgG1
Conjugate APC
Target Species Human
Applications MICS (MACSima Imaging Cyclic Staining), FC, IF, IHC, Mass Cytometry
Description Clone REA175 recognizes CD235a, commonly known as glycophorin A. CD235a is a single-pass transmembrane glycoprotein and is expressed on mature erythrocytes and erythroid precursor cells. It is one of the major sialoglycoprotein expressed on human red blood cells. | The CD235a (Glycophorin A) antibody has been tested by flow cytometric analysis of human peripheral blood cells according to the protocol. Binding of the antibody to red cells at high and moderate antibody concentration can cause cell agglutination, so it is recommended to titrate the antibody carefully for optimal performance in the assay of interest. | Additional information: Clone REA175 displays negligible binding to Fc receptors. |
About CD235a and APC
CD235a Glycophorins A (GYPA) and B (GYPB) are major sialoglycoproteins of the human erythrocyte membrane which bear the antigenic determinants for the MN and Ss blood groups. In addition to the M or N and S or s antigens that commonly occur in all populations, about 40 related variant phenotypes have been identified. These variants include all the variants of the Miltenberger complex and several isoforms of Sta, as well as Dantu, Sat, He, Mg, and deletion variants Ena, S-s-U- and Mk. Most of the variants are the result of gene recombinations between GYPA and GYPB. [provided by RefSeq, Jul 2008]
APC Allophycocyanin (APC) is a fluorescent protein derived from cyanobacteria and red algae and a potent donor fluorophore to create tandem dyes that can be excited off the 633-640 nm laser. APC has an excitation peak at 650 nm and a emission peak at 660 nm.
Citations
PMID 1391951
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APC Excitation and Emission Spectra