FluoroFinder

	Supplier	Miltenyi Biotec
	Catalog #:	130-118-677 (View supplier product page)
	Size	30 tests in 60 µL
	Price	$155.00
	Antigen	LAG-3
	Clone	REA351
	Host	Human
	Isotype	IgG1
	Conjugate	Vio Blue
	Target Species	Human
	Applications	FC, MICS (MACSima Imaging Cyclic Staining), IF, IHC, Mass Cytometry
	Description	Clone REA351 recognizes the human CD223 antigen, a single-pass type I membrane protein also known as lymphocyte activation gene 3 protein (LAG-3). CD223 is a CD4 homolog with four extracellular Ig superfamily domains. Similar to CD4, CD223 oligomerizes at the surfaces of T cells and binds to MHC II on antigen presenting cells but with significantly higher affinity than CD4. CD223 is expressed on activated CD4+ and CD8+ T lymphocytes where it associates with the CD3–TCR complex at the cell surface and negatively regulates signal transduction. As a consequence, it negatively regulates T cell proliferation, function, and homeostasis. There is also increasing evidence of its involvement in regulatory function of tumor-infiltrated T cells in cancer, such as in Hodgkin’s lymphomas and in prostate cancer. CD223 is also expressed on activated NK cells, B cells, and plasmacytoid dendritic cells. \| Additional information: Clone REA351 displays negligible binding to Fc receptors.

	LAG-3	Lymphocyte-activation protein 3 belongs to Ig superfamily and contains 4 extracellular Ig-like domains. The LAG3 gene contains 8 exons. The sequence data, exon/intron organization, and chromosomal localization all indicate a close relationship of LAG3 to CD4. [provided by RefSeq, Jul 2008]
	Vio Blue	Vio®Blue® has an excitation peak at 400 nm and an emission peak at 455 nm, and is spectrally similar to eFluor™ 450 (ThermoFisher Scientific), V450 (BD Biosciences), Alexa Fluor™ 405 (ThermoFisher), Pacific Blue (ThermoFisher Scientific) and ATTO 425 (AttoTec).

$155.00
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LAG-3 / Vio Blue / REA351