FluoroFinder

	Supplier	Miltenyi Biotec
	Catalog #:	130-118-496 (View supplier product page)
	Size	30 tests in 60 µL
	Price	$155.00
	Antigen	CD194
	Clone	REA279
	Host	Human
	Isotype	IgG1
	Conjugate	PE-Vio770
	Target Species	Human
	Applications	FC, Mass Cytometry
	Description	Clone REA279 recognizes the CD194 antigen, a seven-transmembrane G-protein–coupled receptor also known as C-C chemokine receptor type 4 (CCR4). CD194 is expressed on activated Tʜ2 cells, regulatory T cells, a subset of B cells, activated natural killler (NK) cells, basophils, monocytes, NK cells, and platelets. It is expressed at high levels by skin-infiltrating lymphocytes, at lower levels by lung and synovial fluid lymphocytes, but never by intestinal lymphocytes. CD194 is the specific receptor for the chemokines CCL17 (TARC) and CCL22 (MDC). Human peripheral blood regulatory T cells can be divided into two distinct populations based on the expression of CCR4. The majority of freshly isolated regulatory T cells express CD194 and represent memory-type regulatory T cells, while CD194 regulatory T cells require anti-CD3 antibody–mediated activation to acquire a regulatory activity. Depletion of 194+ T cells leads to Tʜ1-type polarization of CD4+ T cells and augmentation of CD8+ T cell responses to tumor antigens. \| Additional information: Clone REA279 displays negligible binding to Fc receptors.

	CD194	The protein encoded by this gene belongs to the G-protein-coupled receptor family . It is a receptor for the CC chemokine - MIP-1, RANTES, TARC and MCP-1. Chemokines are a group of small polypeptide, structurally related molecules that regulate cell trafficking of various types of leukocytes. The chemokines also play fundamental roles in the development, homeostasis, and function of the immune system, and they have effects on cells of the central nervous system as well as on endothelial cells involved in angiogenesis or angiostasis. [provided by RefSeq, Jul 2008]
	PE-Vio770	PE-Vio® 770 from Miltenyi Biotec is a red-emitting tandem fluorophore that combines pycoerythrin (PE) and Vio®770. The donor molecule, PE can be excited by the 488-nm blue, 532-nm green, or 561-nm yellow-green laser and and transfers energy to the acceptor molecule, Vio®770, which emitts light that can be captured with a 780/60 nm bandpass filter. PE-Vio®770 has an excitation peak at 565 nm and an emission peak at 775 nm and is a common alternative to PE-Cy7 and PE-H7.

$155.00
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CD194 / PE-Vio770 / REA279