CD40L / PE-Vio770 / 5C8

Citations: (1)
Product Details
Supplier Miltenyi Biotec
Catalog #: 130-114-134 (View supplier product page)
Size 30 tests in 60 µL
Price $160.00
Antigen CD40L
Clone 5C8
Host Mouse
Isotype IgG2a κ
Conjugate PE-Vio770
Target Species Human
Applications FC
Description The antibody specifically recognizes the human CD154 antigen, a 39 kDa transmembrane glycoprotein, also known as CD40L, gp39, T-BAM, TRAP, or Ly-62. CD154 is transiently up-regulated on activated CD4+ T cells and plays an important role as a costimulatory molecule in T cell/antigen-presenting cell interactions through ligation of CD40. The antibody has been shown to block the activation of antigen-presenting cells by T helper cells in vitro. Because of its transient expression within hours after activation, CD154 can be used as a marker for activated antigen-specific CD4+ T cells. The addition of a CD40-blocking antibody during the stimulation of cell suspensions prevents the down-regulation of CD154 expression induced by interaction with CD40 expressed on antigen-presenting cells. For intracellular detection of CD154 expression or if a pure population of enriched T cells is used, blocking of CD40 is not required.
About CD40L and PE-Vio770
CD40L The protein encoded by this gene is expressed on the surface of T cells. It regulates B cell function by engaging CD40 on the B cell surface. A defect in this gene results in an inability to undergo immunoglobulin class switch and is associated with hyper-IgM syndrome. [provided by RefSeq, Jul 2008]
PE-Vio770 PE-Vio® 770 from Miltenyi Biotec is a red-emitting tandem fluorophore that combines pycoerythrin (PE) and Vio®770. The donor molecule, PE can be excited by the 488-nm blue, 532-nm green, or 561-nm yellow-green laser and and transfers energy to the acceptor molecule, Vio®770, which emitts light that can be captured with a 780/60 nm bandpass filter. PE-Vio®770 has an excitation peak at 565 nm and an emission peak at 775 nm and is a common alternative to PE-Cy7 and PE-H7.
Citations
PMID 16186818
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PE-Vio770 Excitation and Emission Spectra