FluoroFinder

	Supplier	BioLegend
	Catalog #:	909902 (View supplier product page)
	Size	25UL
	Price	$89.00
	Antigen	Myosin heavy chain 10
	Clone	Poly19099
	Host	Rabbit
	Isotype	IgG
	Conjugate	Unconjugated
	Target Species	Human, Mouse, Rat
	Applications	WB, ICC, IHC-P, IHC-Fr
	Description	Purified Non-muscle Myosin Heavy Chain II-B

	Myosin heavy chain 10	This gene encodes a member of the myosin superfamily. The protein represents a conventional non-muscle myosin; it should not be confused with the unconventional myosin-10 (MYO10). Myosins are actin-dependent motor proteins with diverse functions including regulation of cytokinesis, cell motility, and cell polarity. Mutations in this gene have been associated with May-Hegglin anomaly and developmental defects in brain and heart. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]

		Western blot analysis of cell lysates from Hela (positive control), Daudi (low expression negative control) (Human) and UMR106 (Rat) using Non-muscle Myosin Heavy Chain II-B (Myosin-10) Rabbit primary antibody and HRP Donkey anti-Rabbit secondary antibody (Cat. No. 406401). Direct-Blot™ HRP anti-β-actin (Cat. No. 643807) was used as a loading control.
		Immunofluorescence of HeLa cells with (A) Rabbit Isotype control (Cat. No. 910801) or (B-D) Non-muscle Myosin Heavy Chain II-B primary Antibody. Alexa Fluor® 594 (Red) Donkey anti-Rabbit IgG (Cat. No. 406418) was used as secondary antibody. Nuclei were counterstained with DAPI (Blue, Cat. No. 422801). The image was captured with a 60X objective. Exposure time (Seconds) for (A) is 1/30, and (B-D) is 1/60. Concentrations for (A, B) is 2 µg/ml, (C) is 1 µg/ml, (D) is 0.5 µg/ml.