CD90 / Brilliant Violet 650 / 5E10

Product Details
Supplier BioLegend
Catalog #: 328143 (View supplier product page)
Size 25T
Price $209.00
Antigen CD90
Clone 5E10
Host Mouse
Isotype IgG1 κ
Conjugate Brilliant Violet 650
Target Species Human
Applications FC
Description Brilliant Violet 650 anti-human CD90 (Thy1)
About CD90 and Brilliant Violet 650
CD90 This gene encodes a cell surface glycoprotein and member of the immunoglobulin superfamily of proteins. The encoded protein is involved in cell adhesion and cell communication in numerous cell types, but particularly in cells of the immune and nervous systems. The encoded protein is widely used as a marker for hematopoietic stem cells. This gene may function as a tumor suppressor in nasopharyngeal carcinoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2015]
Brilliant Violet 650 Brilliant™ Violet 650 (BV650) is a red-emitting tandem fluorophore that can be excited by the 405 nm Violet laser and collected using a 660/20 bandpass filter. BV650 has an excitation peak at 405 nm and an emission peak at 645 nm. Due to its unique Stokes shift, not many dyes have identical excitation and emission spectrums, but the nearest alternatives include SuperBright 645, Qdot® 655 and eFluor™ 650NC (ThermoFisher). BV650 exhibits a medium level of brightness and is most often used in flow cytometry, specifically, it is an alternative to nanocrystals used for intracellular flow. This dye is part of the Brilliant™ Violet dye line of fluorescent polymers. Brilliant™ Violet 421 polymer is employed as the donor molecule in a series of tandem dyes with acceptor molecules emitting at various points across the visible light spectrum. The Brilliant™ Violet dyes are a superior alternative to QDot nanocrystals and similar to SuperNova dye from Beckman Coulter and StarBright dyes from Bio-Rad.
Images
  CD90 Brilliant Violet 650 BioLegend antibody anti target:Human host:Mouse isotype:IgG1 κ clone:5E10

Human erythroleukemia cell line (HEL) was stained with CD90 (Thy1, clone 5E10) Brilliant Violet 650™ (filled histogram) or Mouse IgG1, κ Brilliant Violet 650™ isotype control (open histogram).

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Brilliant Violet 650 Excitation and Emission Spectra