Perforin / Alexa Fluor 488 / B-D48
Perforin / Alexa Fluor 488 / B-D48
Product Details
| Supplier | BioLegend | |
|---|---|---|
| Catalog #: | 353319 (View supplier product page) | |
| Size | 25T | |
| Price | $136.00 | |
| Antigen | Perforin | |
| Clone | B-D48 | |
| Host | Mouse | |
| Isotype | IgG1 | |
| Conjugate | Alexa Fluor 488 | |
| Target Species | Human | |
| Applications | ICS | |
| Description | Alexa Fluor 488 anti-human Perforin |
About Perforin and Alexa Fluor 488
| Perforin | This gene encodes a protein with structural similarities to complement component C9 that is important in immunity. This protein forms membrane pores that allow the release of granzymes and subsequent cytolysis of target cells. Whether pore formation occurs in the plasma membrane of target cells or in an endosomal membrane inside target cells is subject to debate. Mutations in this gene are associated with a variety of human disease including diabetes, multiple sclerosis, lymphomas, autoimmune lymphoproliferative syndrome (ALPS), aplastic anemia, and familial hemophagocytic lymphohistiocytosis type 2 (FHL2), a rare and lethal autosomal recessive disorder of early childhood. [provided by RefSeq, Aug 2017] | |
|---|---|---|
| Alexa Fluor 488 | Alexa Fluor™ 488 (AF488, Alexa 488) has an excitation peak at 488 nm and an emission peak at 496 nm, and is considered a high-performance alternative to FITC. Alexa 488 is one of the most popular Alexa Fluor™ dyes and is widely used in Fluorescence Microscopy, flow cytometry, and for staining low expression markers. It is bright, highly photostable, resistant to pH changes, and less susceptible to photobleaching. Alexa 488 and is similar in size, brightness and application to DyLight™ 488, iFluor® 488 and CF®488A. |
Images
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Human peripheral blood lymphocytes were surface stained with CD8 APC, fixed with Fixation Buffer, permeabilized with Intracellular Staining Permeabilization Wash Buffer, and then intracellularly stained with Perforin (clone B-D48) Alexa Fluor®488 (left), or mouse IgG1, κ Alexa Fluor®488 isotype control (right). |
Citations
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Reviews & Ratings
Alexa Fluor 488 Excitation and Emission Spectra
$136.00
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