SH3D19 / FITC /

Product Details
Supplier Biorbyt
Catalog #: orb318371-100ug (View supplier product page)
Size 100 μg
Price $451.00
Antigen SH3D19
Clone
Host Rabbit
Isotype IgG
Conjugate FITC
Target Species Human, Mouse, Rat
Applications IF, ICC
Description Rabbit polyclonal antibody against SH3D19 conjugated to FITC.
About SH3D19 and FITC
SH3D19 This gene encodes a multiple SH3 domain-containing protein, which interacts with other proteins, such as EBP and members of ADAM family, via the SH3 domains. This protein may be involved in suppression of Ras-induced cellular transformation and Ras-mediated activation of ELK1 by EBP, and regulation of ADAM proteins in the signaling of EGFR-ligand shedding. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]
FITC Fluorescein isothiocyanate (FITC) has an excitation peak at 495 nm and an emission peak at 519 nm. The name FITC is a misnomer in that the isothiocyanate is a reactive form of this dye. Once FITC is conjugated to an antibody, it is simply Fluorescein conjugated. FITC is one of the most widely used dyes for fluorescent applications, therefore most instruments come standard with a 488 nm laser and FITC filter set up. FITC is commonly conjugated to secondary antibodies and used in applications such as flow cytometry, immunocytochemistry, and immunohistochemistry. FITC is relatively dim, sensitive to photobleaching and it is susceptible to changes is pH. There are better performing alternatives to FITC, like Vio®Bright 515, Alexa Fluor™ 488, iFluor® 488, CF®488A and DY-488. FITC is a long-time generic dye with no sole manufacturer or trademark.
Citations
Additional
Sources
Reviews & Ratings
Submit a Review

FITC Excitation and Emission Spectra