Cav1.2 / ATTO 594 / S57-46

Product Details
Supplier Biorbyt
Catalog #: orb148154-100ug (View supplier product page)
Size 100 μg
Price $575.00
Antigen Cav1.2
Clone S57-46
Host Mouse
Isotype IgG1
Conjugate ATTO 594
Target Species Human, Rat
Applications WB, IHC-P, IF, ICC, IP
Description Mouse monoclonal to CaV1 (ATTO 594). 2. Ion channels are integral membrane proteins that help establish and control the small voltage gradient across the plasma membrane of living cells by allowing the flow of ions down their electrochemical gradient. They are present in the membranes that surround all biological cells because their main function is to regulate the flow of ions across this membrane. Whereas some ion channels permit the passage of ions based on charge, others conduct based on an ionic species, such as sodium or potassium. Furthermore, in some ion channels, the passage is governed by a gate which is controlled by chemical or electrical signals, temperature, or mechanical forces. There are a few main classifications of gated ion channels. There are voltage- gated ion channels, ligand- gated, other gating systems and finally those that are classified differently, having more exotic characteristics. The first are voltage- gated ion channels which open and close in response to membrane potential. These are then separated into sodium, calcium, potassium, proton, transient receptor, and cyclic nucleotide-gated channels; each of which is responsible for a unique role. Ligand-gated ion channels are also known as ionotropic receptors, and they open in response to specific ligand molecules binding to the extracellular domain of the receptor protein. The other gated classifications include activation and inactivation by second messengers, inward-rectifier potassium channels, calcium-activated potassium channels, two-pore-domain potassium channels, light-gated channels, mechano-sensitive ion channels and cyclic nucleotide-gated channels. Finally, the other classifications are based on less normal characteristics such as two-pore channels, and transient receptor potential channels. Specifically, Cav1. 2 is a cardiac L-type calcium channel, and is important for excitation and contraction of the heart. It may be associated with a variant of Long QT syndrome called Timothy's syndrome and also with Brugada syndrome. Some references also suggest it is related to bipolar disease as well....
About Cav1.2 and ATTO 594
Cav1.2 This gene encodes an alpha-1 subunit of a voltage-dependent calcium channel. Calcium channels mediate the influx of calcium ions into the cell upon membrane polarization. The alpha-1 subunit consists of 24 transmembrane segments and forms the pore through which ions pass into the cell. The calcium channel consists of a complex of alpha-1, alpha-2/delta, beta, and gamma subunits in a 1:1:1:1 ratio. There are multiple isoforms of each of these proteins, either encoded by different genes or the result of alternative splicing of transcripts. The protein encoded by this gene binds to and is inhibited by dihydropyridine. Alternative splicing results in many transcript variants encoding different proteins. Some of the predicted proteins may not produce functional ion channel subunits. [provided by RefSeq, Oct 2012]
ATTO 594 ATTO 594 from ATTO-TEC has an excitation peak at 601 nm and an emission peak at 627 nm. ATTO 594 is a novel fluorescent label belonging to the class of rhodamine dyes. Characteristic features of the label are strong absorption, high fluorescence quantum yield, high thermal and photo-stability, and excellent water solubility. Thus ATTO 594 is highly suitable for single-molecule detection applications and high-resolution microscopy such as PALM, dSTORM, STED etc. Additionally the dye highly qualifies to be applied in flow cytometry (FACS), fluorescence in-situ hybridization (FISH) and many more. ATTO 594 is very hydrophilic and shows a very good water solubility. After coupling to a substrate the label carries a net electrical charge of -1.
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ATTO 594 Excitation and Emission Spectra