University of Pittsburgh
Dietrich School of Arts & Sciences, Microscopy Facility

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Attune NxT Configuration

Laser (nm) BP SP LP
Optimal Colors
Additional optimal fluorochrome options available on FluoroFinder.com.
Laser

405

440 / 50

495

417

 Alexa Fluor 405
Brilliant Violet 421
Brilliant Violet 480
Calcein Violet AM
CellTrace Violet
DY-415
DyLight 405
eFluor 450
Vio Blue
Vybrant DyeCycle Violet

512 / 25

555

495

 CFP
eFluor 506
Live/Dead Fix Aqua
Pacific Green
Alexa Fluor 430
AmCyan
Brilliant Violet 510
DY-380XL
Krome Orange
Pacific Orange

603 / 48

650

555

 eVolve 605
Live/Dead Fix Yellow
Pacific Orange
Qdot 605
SuperBright 600
Alexa Fluor 430
Brilliant Violet 570
Brilliant Violet 605
DyLight 405LS
eFluor 605NC

710 / 50

--

650

 Qdot 705
SuperBright 702
Laser

488

530 / 30

555

503

 Alexa Fluor 488
CFSE
FITC
GFP
Sytox Green
ATTO 488
Brilliant Blue 515
CF488A
CF503R
Cy2

590 / 40

650

555

 Live/Dead Fix Red
PE-Texas Red
Propidium Iodide
ECD
PE-CF594
PE-Dazzle 594
PE-eFluor 610
PE-Vio615

695 / 40

--

650

 PE-Alexa 700
PerCP
PerCP-Cy5.5
Brilliant Blue 700
PerCP-eFluor 710
PerCP-Vio700
Laser

561

585 / 16

600

577

 PE
Propidium Iodide
RFP
Sytox Orange
CF550R
CF568
CF570

620 / 15

650

600

 mCherry
PE-Alexa 610
PE-Texas Red
Propidium Iodide
Sytox AADvanced
CF583
CF583R
DyLight 594
ECD
PE-CF594

695 / 40

740

650

 PE-Alexa 700
PE-Cy5.5
PerCP-Cy5.5

780 / 60

--

740

 PE-Cy7
PE-Vio770
Laser

640

670 / 14

690

654

 Alexa Fluor 647
APC
eFluor 660
Live/Dead Fix Far Red
Sytox Red
Alexa Fluor 633
Alexa Fluor 635
CF640R
CF647
Cy5

720 / 30

740

690

 Alexa Fluor 680
Alexa Fluor 700
APC-Cy5.5
APC-R700
DyLight 680

780 / 60

--

740

 APC-Alexa 750
APC-Cy7
APC-eFluor 780
APC-H7
Live/Dead Fix Near IR (780)
Vybrant DyeCycle Ruby
APC-Fire 750
APC-Vio770

Highlighted colors are suggest by the instrument owner or manufacturer.

Tip: FluoroFinder.com enables interactive product selection from hundreds of antibody suppliers with machine specific spectra-viewers to guide you to an optimal panel design.