Aria Assembly 5 Laser G536 Configuration

	Laser (nm)	LP	Optimal Colors Additional optimal fluorochrome options available on FluoroFinder.com.
Laser	355	740 / 35	690	Brilliant Ultraviolet 737 StarBright UltraViolet 740
Laser	355	379 / 28	--	Brilliant Ultraviolet 395 mFluor UV 375 Spark UV 387 StarBright UltraViolet 400

Laser	405	780 / 60	750	Aco-800 Brilliant Violet 750 Brilliant Violet 785 Brilliant Violet 786 StarBright Violet 760 StarBright Violet 790 SuperBright 780 SuperNova v786 Qdot 800
		710 / 50	690	Brilliant Violet 711 Brilliant Violet 750 StarBright Violet 710 StarBright Violet 760 SuperBright 702 Qdot 705
		660 / 20	630	Brilliant Violet 650 Chlorophyll A StarBright Violet 670 SuperBright 645 eFluor 650NC eVolve 655 Qdot 655
		525 / 50	505	Aco-490 ATTO 425 ATTO 430LS Brilliant Violet 480 Brilliant Violet 510 CytoCalcein Violet 500 DY-395XL LipidSpot 488 LysoBrite Blue LysoSensor Yellow/Blue pH3.0 MitoLite Blue FX490 MitoLite Orange EX405 Nuclear Yellow Spark Violet 538 Spectrum Aqua StarBright Violet 475 StarBright Violet 515 Vio Green
		450 / 50	--	AbBy Fluor 405 ABflo 405 ABflo 450 Aco-490 Alexa Fluor 405 ATTO 425 Brilliant Violet 421 Brilliant Violet 480 CF405M CF405S cFluor V420 cFluor V450 CoraLite Plus 405 CytoCalcein Violet 450 CytoCalcein Violet 500 DY-415 DyLight 405 eFluor 450 ExoBrite 410/450 EV Membrane Stain HiLyte 405 iFluor 405 LysoBrite Blue LysoView 405 MaxLight 405 MemBrite Fix 405/430 mFluor Violet 450 MitoLite Blue FX490 MitoView 405 Nuclear Violet Nuclear Yellow Oxazole Blue Homodimer Pacific Blue POPO-1 PromoFluor 405 RayBright Violet 450 Spark Violet 423 Spectrum Aqua StarBright Violet 440 StarBright Violet 475 SuperBright 436 SuperNova v428 V450 Vio Blue Vio Bright V423 violetFluor 450

Laser	488	710 / 50	685	ATTO 490LS Brilliant Blue 700 cFluor B690 cFluor BYG680 cFluor BYG710 Di-8-ANEPPS NovaFluor Blue 660 / 40S NovaFluor Blue 690 PE-Cy5 PE-Cy5.5 PE-Dyomics 647 PE-Fire 700 PerCP-eFluor 710 PerCP-Vio700 PE-Vio670 RealBlue 705 RealBlue 744 RPE-CF647T StarBright Blue 675 StarBright Blue 700 StarBright Blue 765 SureLight PE Cyanine 5.5
Laser	488	530 / 30	505	AbBy Fluor 488 ABflo 488 Adirondack Green 520 Alexa Fluor 488 Alpha Fluor 488 ATTO 488 BactoView Dead 500/515 BactoView Live Green BCECF AM Brilliant Blue 515 CellTrace Oregon Green CellTracker Green CF488A CF500 cFluor B515 cFluor B532 cFluor B548 Chromeo 488 Chromeo 505 CoraLite 532 CoraLite Plus 488 CytoTrace Green CMFDA DAF-FM DMAO DyLight 488 ExoBrite 490/515 EV Membrane Stain FITC FITC Plus HiLyte 488 iFluor 488 KIRAVIA Blue 520 LysoBrite Green LysoView 488 MaxLight 490 MemBrite Fix 488/515 MitoLite Green EX488 MitoLite Green FM MitoMark Green MitoROS 520 NBD Neuro DiO NL 493 NovaFluor Blue 530 Nuclear Green Nuclear Orange NucSpot 470 NucSpot Live 488 Oxazole Yellow Oxazole Yellow Homodimer Oyster 488 PhenoVue 493 Lipid Stain PhenoVue 503 Lysosomal Stain PhenoVue Cal-520 AM Bright, Calcium Indicator PhenoVue Cal-520 AM, Calcium Indicator PhenoVue Fluo-4 AM, Calcium Indicator PhenoVue Fluor 488 PhenoVue ROS-490, Total Oxidative Stress Indicator PicoGreen PromoFluor 488P RayBright Blue 488 RealBlue 545 Spark Blue 550 Spark Blue 574 Spectrum Green SureLight 488 Syto 16 Syto 24 Thiazole Orange Homodimer Thiazole Orange Iodide Vio 488 Vio 515 Vio Bright 515 Vio Bright FITC VRDye 490 XFD488

Laser	552	780 / 60	750	DRAQ 9 NovaFluor Yellow 755 PE-Cy7 PE-Fire 810 PE-Vio770 RayBright PE-Cy7 RealYellow 775 Seta-R-PE-775 StarBright Yellow 800 SureLight PE Cyanine 7.0
		710 / 50	685	BactoView Live Red DRAQ 9 LysoView 594 mNeptune NovaFluor Yellow 660 NovaFluor Yellow 755 NucSpot Far-Red PE-Cy5 PE-Cy5.5 PE-Dyomics 647 PE-Fire 700 PE-Vio670 RealYellow 703 RPE-CF647T StarBright Yellow 665 StarBright Yellow 720 SureLight PE Cyanine 5.5
		610 / 20	600	ATTO 550 ATTO 565 ATTO Rho3B BOBO-3 Calcein Red-Orange AM CF568 CF570 DyLight 549 ExoBrite 560/585 EV Membrane Stain LysoTracker Red LysoView 550 LysoView 594 MemBrite Fix 568/580 MitoLite Orange FX570 mNeptune mScarlet NovaFluor Yellow 610 PE-Alexa 610 PE-ATTO 594 PE-CF594 PE-Dazzle 594 PE-DyLight 594 PE-Dyomics 590 PE-eFluor 610 PE-Vio615 PhenoVue Nile Red Lipid Stain RealYellow 586 RealYellow 610 ReAsH Rhodamine Red Rhod-FF AM SNARF 1 pH6.0 SNARF 1 pH9.0 Spark YG 581 StarBright Yellow 605 SureLight PE-594
		575 / 26	--	AbBy Fluor 555 ABflo 555 Alexa Fluor 546 Alexa Fluor 555 Alexa Fluor 561 ATTO 542 ATTO 550 ATTO 565 ATTO Rho3B BOBO-3 Calcein Red AM Calcein Red-Orange AM Calcium Orange CellMask Orange Plasma Membrane Stain CF568 CF570 CytoTrace Red DyLight 549 DyLight 550 Dyomics 547 ExoBrite 560/585 EV Membrane Stain HiLyte 555 iFluor 532 LysoBrite Orange LysoTracker Red LysoView 550 MemBrite Fix 543/560 MemBrite Fix 568/580 mFluor Blue 570 MitoLite Orange FX570 mScarlet NL 557 NovaFluor Yellow 570 PE PE-Dyomics 590 PhenoVue 551 Mitochondrial Stain PhenoVue Fluor 555 PhenoVue ROS-570, Total Oxidative Stress Indicator POPO-3 RayBright PE RealYellow 586 Rhodamine Red Rhod-FF AM SNARF 1 pH6.0 Spark YG 581 XFD555

Laser	635	780 / 60	750	APC-CF750 APC-CF770 APC-eFluor 780 APC-Fire 750 APC-Fire 810 APC-H7 APC-Vio770 DRAQ 9 LysoView 680 NovaFluor Red 755 PE-Fire 780 RayBright Red 780
		730 / 45	690	Alexa Fluor 660 APC-Alexa 680 APC-Alexa 700 APC-CF750 APC-R700 ATTO 647 ATTO 655 DRAQ 9 IRDye 680RD LipidSpot 610 LysoView 650 LysoView 680 MitoLite NIR FX690 NovaFluor Red 725 NovaFluor Red 755 NucSpot 680 NucSpot Far-Red NucSpot Live 650 PE-Fire 780 Red 718 Seta-APC-705 Spark NIR 685 Vio Bright R720 Vio R720
		670 / 30	--	AbBy Fluor 647 ABflo 647 Alexa Fluor 633 Alexa Fluor 635 Alexa Fluor 647 Alexa Fluor 660 APC ATTO 620 ATTO 633 ATTO 647 ATTO 647N ATTO 655 Calcein Deep Red AM CellTracker Deep Red CellVue Claret CF633 CF640R CF647 cFluor R685 Chromeo 642 CoraLite 647A CoraLite Plus 647 Cy5 CypHer5E DRAQ 9 DyLight 633 DyLight 649 DyLight 650 ExoBrite 640/660 EV Membrane Stain FluoroProbes647h HiLyte 647 iFluor 633 iFluor 647 IRDye 650 IRDye 680RD Janelia Fluor 635 Janelia Fluor 646 LipidSpot 610 LysoBrite NIR LysoTracker Deep Red LysoView 640 LysoView 650 MaxLight 650 MemBrite Fix 640/660 MemBrite Fix ST 650/665 MitoLite Deep Red FX660 MitoLite NIR FX690 MitoView 650 MitoView Fix 640 NL 637 NucSpot 640 NucSpot Far-Red NucSpot Live 650 Oyster 650 PhenoVue 641 Mitochondrial Stain PhenoVue Fluor 647 PhenoVue ROS-670, Total Oxidative Stress Indicator PromoFluor 647P RayBright APC RayBright Red 647 Spark NIR 685 STAR 635 STAR 635P STAR RED SureLight APC SureLight P3 SureLight RPC Thiazole Red Thiazole Red Homodimer Vio 667 Vio Bright 667 Vio Bright R667 Vio R667 XFD647

Highlighted colors are suggest by the instrument owner or manufacturer.

Tip: FluoroFinder.com enables interactive product selection from hundreds of antibody suppliers with machine specific spectra-viewers to guide you to an optimal panel design.

University of Pittsburgh Unified Flow Core

Aria Assembly 5 Laser G536 Configuration

355

740 / 35

690

379 / 28

--

405

780 / 60

750

710 / 50

690

660 / 20

630

525 / 50

505

450 / 50

--

488

710 / 50

685

530 / 30

505

552

780 / 60

750

710 / 50

685

610 / 20

600

575 / 26

--

635

780 / 60

750

730 / 45

690

670 / 30

--

University of Pittsburgh
Unified Flow Core