University of Pittsburgh
Unified Flow Core

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Aria 5 laser BST E1514 Configuration

Laser (nm) BP LP
Optimal Colors
Additional optimal fluorochrome options available on FluoroFinder.com.
Laser

355

440 / 40

--

 Hoechst (33258)
Indo-Lo (Ca2+free)

530 / 30

505

 Hoechst (33342)
Indo-Hi (Ca2+bound)
Laser

405

450 / 50

--

 Alexa Fluor 405
DAPI
eFluor 450 Fix Viability
Pacific Blue
V450
Brilliant Violet 421
CF405M
DyLight 405
eFluor 450
Vio Blue

525 / 50

505

 AmCyan
Alexa Fluor 430
Brilliant Violet 510
V500
Vio Green

605 / 40

595

 Brilliant Violet 605
DyLight 405LS
eFluor 605NC
eVolve 605
Qdot 605
SuperBright 600
Laser

488

515 / 20

505

 CFSE
FITC
GFP
PKH67
Alexa Fluor 488
Brilliant Blue 515
CF488A
CF503R
Cy2
DyLight 488

695 / 40

685

 EMA
PerCP
PerCP-Cy5.5
Propidium Iodide
Brilliant Blue 700
PerCP-eFluor 710
PerCP-Vio700
Laser

532

575 / 26

--

 PE
PKH26
Pyronin Y
CF550R
CF570

610 / 20

600

 dsRed
ECD
PE-Texas Red
Propidium Iodide
PE-CF594
PE-Dazzle 594
PE-eFluor 610
PE-Vio615

660 / 40

640

 PE-Cy5
7-AAD

710 / 50

690

 PE-Cy5.5

780 / 40

740

 PE-Cy7
PE-Vio770
Laser

635

660 / 20

--

 Alexa Fluor 647
APC
Alexa Fluor 633
Alexa Fluor 635
CF640R
CF647
Cy5
DyLight 633
DyLight 650
NL 637

730 / 45

710

 Alexa Fluor 700
DRAQ 5
APC-R700

780 / 60

735

 APC-Alexa 750
APC-Cy7
APC-eFluor 780
APC-H7
APC-Fire 750
APC-Vio770

Highlighted colors are suggest by the instrument owner or manufacturer.

Tip: FluoroFinder.com enables interactive product selection from hundreds of antibody suppliers with machine specific spectra-viewers to guide you to an optimal panel design.