University of Pittsburgh
Unified Flow Core

Design a panel and find antibodies today at

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LSR II BST E1514 Configuration

Laser (nm) BP LP
Optimal Colors
Additional optimal fluorochrome options available on FluoroFinder.com.
Laser

355

379 / 28

--

 Brilliant Ultraviolet 395
DAPI
Indo-Hi (Ca2+bound)

740 / 35

690

 Brilliant Ultraviolet 737
DAPI
Hoechst (33258)
Laser

405

450 / 50

--

 Alexa Fluor 405
DAPI
eFluor 450 Fix Viability
Marina Blue
Pacific Blue
V450
Brilliant Violet 421
CF405M
DyLight 405
eFluor 450

525 / 50

505

 Alexa Fluor 430
AmCyan
CFP
Brilliant Violet 510
DyLight 405LS
Krome Orange
Pacific Orange
V500
Vio Green

610 / 20

550

 Brilliant Violet 605
DyLight 405LS
eFluor 605NC
eVolve 605
Qdot 605
SuperBright 600
Laser

488

515 / 50

505

 CFSE
FITC
GFP
YFP
Alexa Fluor 488
Brilliant Blue 515
CF488A
CF503R
Cy2
DyLight 488

695 / 40

635

 PE-Cy5
PerCP
PerCP-Cy5.5
Brilliant Blue 700
PerCP-eFluor 710
PerCP-Vio700
Laser

532

575 / 26

--

 Cy3
PE
Alexa Fluor 546
Alexa Fluor 555
CF550R
CF570
Cyanine 3 SE

610 / 20

595

 dsRed
EMA
PE-Texas Red
Propidium Iodide
ECD
PE-CF594
PE-Dazzle 594
PE-eFluor 610
PE-Vio615

670 / 30

635

 PE-Cy5

710 / 50

685

 PE-Cy5.5

780 / 60

750

 PE-Cy7
PE-Vio770
Laser

640

670 / 14

--

 Alexa Fluor 647
APC
Cy5
Alexa Fluor 633
Alexa Fluor 635
CF640R
CF647
Cyanine 5 SE
DyLight 633
DyLight 650

710 / 50

685

 Alexa Fluor 660
Alexa Fluor 700
APC-R700

780 / 60

750

 APC-Cy7
APC-H7
APC-Alexa 750
APC-eFluor 780
APC-Fire 750
APC-Vio770

Highlighted colors are suggest by the instrument owner or manufacturer.

Tip: FluoroFinder.com enables interactive product selection from hundreds of antibody suppliers with machine specific spectra-viewers to guide you to an optimal panel design.