University of Pittsburgh
Unified Flow Core

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Fortessa Assembly G530 Configuration

Laser (nm) BP LP
Optimal Colors
Additional optimal fluorochrome options available on FluoroFinder.com.
Laser

355

379 / 28

--

 Brilliant Ultraviolet 395
DAPI
Indo-Hi (Ca2+bound)

730 / 45

685

 Brilliant Ultraviolet 737
Hoechst (33258)
Indo-Lo (Ca2+free)
Laser

405

450 / 50

--

 Brilliant Violet 421
Live/Dead Fix Violet
Pacific Blue
V450
Alexa Fluor 405
CF405M
DyLight 405
eFluor 450
SuperBright 436
Vio Blue

525 / 50

505

 Brilliant Violet 510
CFP
V500
Alexa Fluor 430
CF405L
CF430
CF440
DyLight 405LS
Vio Green

610 / 20

595

 Brilliant Violet 605
DyLight 405LS
eFluor 605NC
eVolve 605
Qdot 605
SuperBright 600
Laser

488

530 / 30

505

 FITC
GFP
Alexa Fluor 488
Brilliant Blue 515
CF488A
CF503R
Cy2
DyLight 488
Fluorescein
Vio Bright FITC

670 / 30

655

 PerCP

710 / 50

685

 PerCP-Cy5.5
Brilliant Blue 700
PerCP-eFluor 710
PerCP-Vio700
Laser

532

575 / 26

--

 PE
tdTomato
CF550R
CF570

610 / 20

600

 dsRed
ECD
EMA
mCherry
PE-Texas Red
Propidium Iodide
PE-CF594
PE-Dazzle 594
PE-eFluor 610
PE-Vio615

670 / 30

635

 PE-Cy5
7-AAD

695 / 40

670

 PE-Cy5.5

780 / 60

750

 PE-Cy7
PE-Vio770
Laser

633

670 / 30

--

 Alexa Fluor 647
Alexa Fluor 633
Alexa Fluor 635
APC
CF640R
CF647
Cy5
DyLight 650
NL 637

720 / 40

685

 Alexa Fluor 680
Alexa Fluor 700
APC-Cy5.5
APC-R700
DyLight 680

780 / 60

750

 Alexa Fluor 790
APC-Cy7
APC-H7
APC-Alexa 750
APC-eFluor 780
APC-Fire 750
APC-Vio770

Highlighted colors are suggest by the instrument owner or manufacturer.

Tip: FluoroFinder.com enables interactive product selection from hundreds of antibody suppliers with machine specific spectra-viewers to guide you to an optimal panel design.