University of Oslo
Faculty of Medicine Flow Cytometry Core Facility

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BD FACS Symphony A5 Configuration

Laser (nm) BP LP
Optimal Colors
Additional optimal fluorochrome options available on FluoroFinder.com.
Laser

355

379 / 28

--

 Brilliant Ultraviolet 395

515 / 30

450

 Brilliant Ultraviolet 496

580 / 20

550

 Brilliant Ultraviolet 563

605 / 20

595

 Qdot 605
eVolve 605

670 / 30

630

 Brilliant Ultraviolet 661

735 / 30

690

 Brilliant Ultraviolet 737

810 / 40

770

 Brilliant Ultraviolet 805
Laser

405

431 / 28

--

 Alexa Fluor 405
Brilliant Violet 421
Pacific Blue
CF405M
DyLight 405
eFluor 450
SuperBright 436
V450
Vio Blue

525 / 50

505

 Brilliant Violet 480
Pacific Orange
Qdot 525
Alexa Fluor 430
Brilliant Violet 510
DyLight 405LS
Krome Orange
V500
Vio Green

586 / 15

550

 Brilliant Violet 570
DyLight 405LS
Qdot 585

605 / 40

595

 Brilliant Violet 605
Qdot 605
DyLight 405LS
eFluor 605NC
eVolve 605
SuperBright 600

677 / 20

635

 Brilliant Violet 650
eFluor 650NC
eVolve 655

710 / 50

685

 Brilliant Violet 711
Qdot 705
SuperBright 702

750 / 30

735

 Brilliant Violet 750

810 / 40

770

 Brilliant Violet 785
Brilliant Violet 786
Qdot 800
Laser

488

530 / 30

505

 Brilliant Blue 515
FITC
Alexa Fluor 488
CF488A
CF503R
Cy2
DyLight 488
Fluorescein
Vio Bright FITC

610 / 20

600

 Brilliant Blue 630-P2

710 / 50

685

 Brilliant Blue 700
PerCP-Cy5.5

750 / 30

735

 Brilliant Blue 755-P

810 / 40

770

 Brilliant Blue 790-P
Laser

561

586 / 15

--

 PE
CF550R
CF568
CF570

610 / 20

600

 Alexa Fluor 594
mCherry
PE-CF594
PE-Texas Red
Propidium Iodide
BODIPY TR
CF583
CF583R
DyLight 594
ECD

670 / 30

635

 PE-Cy5

710 / 50

685

 PE-Cy5.5

780 / 60

735

 PE-Cy7
7-AAD
PE-Vio770
Laser

637

670 / 30

--

 Alexa Fluor 647
APC
Alexa Fluor 633
Alexa Fluor 635
CF640R
CF647
Cy5
DyLight 633
DyLight 650
NL 637

730 / 45

690

 Alexa Fluor 700
APC-Alexa 680
APC-R700

780 / 60

750

 APC-H7
APC-Alexa 750
APC-Cy7
APC-eFluor 780
APC-Fire 750
APC-Vio770

Highlighted colors are suggest by the instrument owner or manufacturer.

Tip: FluoroFinder.com enables interactive product selection from hundreds of antibody suppliers with machine specific spectra-viewers to guide you to an optimal panel design.