University of Oslo
Faculty of Medicine Flow Cytometry Core Facility

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(Montebello) LSR II Yellow laser Configuration

Laser (nm) BP LP
Optimal Colors
Additional optimal fluorochrome options available on FluoroFinder.com.
Laser

405

450 / 50

--

 Alexa Fluor 405
Brilliant Violet 421
eFluor 450
Hoechst (33258)
Hoechst (33342)
Pacific Blue
SuperBright 436
V450
CF405M
DyLight 405

525 / 50

505

 Alexa Fluor 430
AmCyan
Brilliant Violet 510
Hoechst (33258)
Hoechst (33342)
Live/Dead Fix Aqua
Pacific Orange
Qdot 525
V500
CF405L

585 / 42

545

 Brilliant Violet 570
Hoechst (33258)
Hoechst (33342)
Qdot 585
DyLight 405LS
Qdot 565

610 / 20

600

 Brilliant Violet 605
Qdot 605
SuperBright 600
DyLight 405LS
eFluor 605NC
eVolve 605

660 / 20

635

 Brilliant Violet 650
Qdot 655
SuperBright 645
eFluor 650NC
eVolve 655

710 / 50

685

 Brilliant Violet 711
Qdot 705
SuperBright 702

780 / 60

735

 Brilliant Violet 785
Brilliant Violet 786
Qdot 800
Laser

488

525 / 50

505

 Alexa Fluor 488
CFSE
FITC
GFP
YFP
ATTO 488
Brilliant Blue 515
CF488A
CF503R
Cy2

685 / 35

635

 PerCP
PerCP-Cy5.5
PerCP-eFluor 710
Brilliant Blue 700
PerCP-Vio700
Laser

561

582 / 15

--

 PE
CF550R
CF568
CF570

610 / 20

600

 mCherry
PE-CF594
PE-Texas Red
Propidium Iodide
CF583
CF583R
DyLight 594
ECD
PE-Dazzle 594
PE-eFluor 610

670 / 14

630

 PE-Cy5
7-AAD

710 / 50

685

 PE-Cy5.5
7-AAD

780 / 60

735

 PE-Cy7
PE-Vio770
Laser

633

670 / 14

--

 Alexa Fluor 647
APC
Alexa Fluor 633
Alexa Fluor 635
CF640R
CF647
Cy5
DyLight 633
DyLight 650
NL 637

730 / 45

685

 Alexa Fluor 680
Alexa Fluor 700
APC-Cy5.5
APC-R700
DyLight 680

780 / 60

735

 Alexa Fluor 750
APC-Cy7
APC-eFluor 780
APC-H7
Zombie NIR
APC-Alexa 750
APC-Fire 750
APC-Vio770

Highlighted colors are suggest by the instrument owner or manufacturer.

Tip: FluoroFinder.com enables interactive product selection from hundreds of antibody suppliers with machine specific spectra-viewers to guide you to an optimal panel design.