University of Oslo
Faculty of Medicine Flow Cytometry Core Facility

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(Montebello) LSR II UV laser Configuration

Laser (nm) BP LP
Optimal Colors
Additional optimal fluorochrome options available on FluoroFinder.com.
Laser

355

450 / 50

--

 Alexa Fluor 350
Brilliant Ultraviolet 395
Brilliant Ultraviolet 496
Hoechst (33258)
Hoechst (33342)
CF350

610 / 20

505

 Qdot 605
eVolve 605
Laser

405

450 / 50

--

 Alexa Fluor 405
Brilliant Violet 421
Hoechst (33258)
Hoechst (33342)
Pacific Blue
SuperBright 436
CF405M
DyLight 405
eFluor 450
V450

530 / 30

505

 Alexa Fluor 430
Hoechst (33258)
Hoechst (33342)
Qdot 525
Brilliant Violet 510
DyLight 405LS
Krome Orange
Pacific Orange
V500
Vio Green

780 / 60

735

 Brilliant Violet 786
Qdot 800
Laser

488

530 / 30

505

 Alexa Fluor 488
CFSE
FITC
ATTO 488
Brilliant Blue 515
CF488A
CF503R
Cy2
DyLight 488
Fluorescein

575 / 26

550

 PE

610 / 20

600

 PE-Texas Red
Propidium Iodide
ECD
PE-CF594
PE-Dazzle 594
PE-eFluor 610
PE-Vio615

670 / 14

635

 PE-Cy5
PerCP-Vio700

695 / 40

685

 PE-Cy5.5
PerCP
PerCP-Cy5.5
7-AAD
Brilliant Blue 700
PerCP-eFluor 710
PerCP-Vio700

780 / 60

755

 PE-Cy7
PE-Vio770
Laser

633

660 / 20

--

 Alexa Fluor 633
Alexa Fluor 647
APC
Alexa Fluor 635
CF640R
CF647
Cy5
DyLight 633
DyLight 650
NL 637

730 / 45

710

 Alexa Fluor 680
Alexa Fluor 700
APC-Cy5.5
APC-R700
DyLight 680

780 / 60

755

 APC-Alexa 750
APC-Cy7
APC-eFluor 780
APC-Fire 750
APC-H7
APC-Vio770

Highlighted colors are suggest by the instrument owner or manufacturer.

Tip: FluoroFinder.com enables interactive product selection from hundreds of antibody suppliers with machine specific spectra-viewers to guide you to an optimal panel design.