University College London
UCL GOSICH Flow Cytometry Core Facility

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FACSCalibur 488/635) Configuration

Laser (nm) BP SP LP
Optimal Colors
Additional optimal fluorochrome options available on FluoroFinder.com.
Laser

488

530 / 30

560

--

 Alexa Fluor 488
Brilliant Blue 515
CFSE
FITC
GFP
YFP
ATTO 488
CF488A
CF503R
Cy2

585 / 42

640

--

 PE

--

--

670

 PE-Cy5
PE-Cy5.5
PE-Cy7
PerCP
PerCP-Cy5.5
PE-Vio770
Propidium Iodide
Brilliant Blue 700
PE-Alexa 750
PerCP-eFluor 710
Laser

635

661 / 16

--

--

 Alexa Fluor 647
APC
TO-Pro 3
Alexa Fluor 633
Alexa Fluor 635
CF640R
CF647
Cy5
DyLight 633
NL 637

Highlighted colors are suggest by the instrument owner or manufacturer.

Tip: FluoroFinder.com enables interactive product selection from hundreds of antibody suppliers with machine specific spectra-viewers to guide you to an optimal panel design.