University College London
UCL GOSICH Flow Cytometry Core Facility

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FACSAria III Cell Sorter (405/488/561/633) Configuration

Laser (nm) BP LP
Optimal Colors
Additional optimal fluorochrome options available on FluoroFinder.com.
Laser

405

450 / 40

--

 BFP
Brilliant Violet 421
Pacific Blue
V450
Alexa Fluor 405
CF405M
DyLight 405
eFluor 450
SuperBright 436
Vio Blue

525 / 50

502

 AmCyan
Brilliant Violet 510
CFP
Qdot 525
Alexa Fluor 430
CF405L
CF430
CF440
DyLight 405LS
V500

585 / 42

575

 Pacific Orange
Qdot 585
Alexa Fluor 430
Brilliant Violet 570
DyLight 405LS
Krome Orange

610 / 20

595

 Brilliant Violet 605
Qdot 605
DyLight 405LS
eFluor 605NC
eVolve 605
SuperBright 600

660 / 20

630

 Brilliant Violet 650
Qdot 655
eFluor 650NC
eVolve 655
SuperBright 645

710 / 50

685

 Brilliant Violet 711
Qdot 705
SuperBright 702

780 / 60

735

 Brilliant Violet 785
Brilliant Violet 786
Qdot 800
Laser

488

530 / 20

502

 Alexa Fluor 488
Brilliant Blue 515
CFSE
FITC
GFP
YFP
ATTO 488
CF488A
CF503R
Cy2

695 / 40

655

 PerCP
PerCP-Cy5.5
PerCP-Vio700
7-AAD
Brilliant Blue 700
PE-Cy5
PE-Cy5.5
PerCP-eFluor 710
Laser

561

582 / 15

--

 PE
CF550R
CF568
CF570

610 / 20

600

 PE-Texas Red
Propidium Iodide
CF583
CF583R
DyLight 594
ECD
PE-CF594
PE-Dazzle 594
PE-eFluor 610
PE-Vio615

670 / 14

630

 PE-Cy5
7-AAD

710 / 50

685

 PE-Cy5.5

780 / 60

735

 PE-Cy7
PE-Vio770
PE-Alexa 750
Laser

633

660 / 20

--

 Alexa Fluor 647
APC
TO-Pro 3
Alexa Fluor 633
Alexa Fluor 635
CF640R
CF647
Cy5
DyLight 633
DyLight 650

730 / 45

690

 Alexa Fluor 700
APC-R700

780 / 60

755

 APC-Cy7
APC-Vio770
APC-Alexa 750
APC-eFluor 780
APC-Fire 750
APC-H7

Highlighted colors are suggest by the instrument owner or manufacturer.

Tip: FluoroFinder.com enables interactive product selection from hundreds of antibody suppliers with machine specific spectra-viewers to guide you to an optimal panel design.