OMIP-009
Characterization of antigen-specific human T-cells
Cytometry PART A, Volume 81A, Issue 5, 363-363 (2012)
Laurie Lamoreaux, Richard A. Koup, Mario Roederer
Description: The panels described in this article are designed to characterize the immunological response of human T-cells to vaccination by measuring the frequency, phenotype, and function of CD4 and CD8 T-cells. Subsequent qualification of the panel allows for comparison of intra- and inter-laboratory outcomes between different vaccine trials such that those vaccine formulations that reveal a possible correlate of protection against infection may be moved forward in the regulatory process. Although the panel was developed for batch analysis of cryopreserved PBMC samples, the assay may also be performed with fresh cells.
Species: Human
Tissue: Peripheral blood
Cell Subset: T cell, T helper, T memory, Ctl
Keyword: Antigen-specific t cell response, Cryopreserved pbmc, Vaccination
Machine: LSR II
Panel details
| Marker | Species | Clone | Fluorophore | Purpose | Co-expressions groups | Antigen Density |
|---|---|---|---|---|---|---|
| CCR7 | Human | 150503 | Alexa Fluor 680 | memory/differentiation | T cell, T memory | Low |
| Dead cells | Human | N/A | Live/Dead Fix Violet | viability | ||
| CD45RA | Human | L48 | PE-Cy7 | memory/differentiation | T cell, T memory | Med |
| CD4 | Human | T4 | ECD | lineage | T cell, T helper, T memory | High |
| IFN-gamma | Human | B27 | APC | function | T cell, T helper | High |
| IL-2 | Human | MQ1-17H12 | PE | function | T cell, T helper | Med |
| CD8a | Human | RPA-T8 | Pacific Blue | lineage | T cell, T memory | High |
| TNF | Human | Mab11 | FITC | function | T cell, T helper | High |
| CD28 | Human | CD28.2 | PE-Cy5 | memory/differentiation | T cell, T memory | Med |
| CD3 | Human | SP34-2 | APC-Cy7 | lineage | T cell, T memory, T helper | High |
