OMIP-008
Measurement of Th1 and Th2 cytokine polyfunctionality of human T cells
Cytometry PART A, Volume 81A, Issue 6, 450-452 (2012)
Cindy L. Zuleger, Mark R. Albertini
Description: This panel was optimized to assess CD4+ and CD8+ T cell responses to various tumor antigens from melanoma patients. The panel was tested on single-cell derived T cell isolates (SCD-T) and T cell lines derived from peripheral blood mononuclear cells (PBMC) from melanoma patients, T cell lines from the tumor environment of melanoma patients, and fresh and cryopreserved PBMC (healthy donors). Staining can be performed in 96-well plates for high-throughput.
Species: Human
Tissue: Cell line, Peripheral blood
Cell Subset: T cell, T helper, Ctl
Keyword: Cryopreserved pbmc, Melanoma
Machine: LSR II
Panel details
| Marker | Species | Clone | Fluorophore | Purpose | Co-expressions groups | Antigen Density |
|---|---|---|---|---|---|---|
| CD19 | Human | HIB19 | eFluor 450 | exclusion | Low | |
| Dead cells | Human | Live/Dead Fix Violet | viability(dump) | |||
| CD4 | Human | RPA-T4 | APC-Cy7 | lineage | T helper | High |
| CD14 | Human | M5E2 | Pacific Blue | exclusion | High | |
| IL-4 | Human | 8D4-8 | Alexa Fluor 488 | function/cytokine secretion | T helper | Low |
| IL-10 | Human | JES3-9D7 | PE | function/cytokine secretion | T helper | Low |
| IFN-gamma | Human | B27 | APC | function/cytokine secretion | T helper | High |
| CD8a | Human | 3B5 | Qdot 605 | lineage | High | |
| TNF | Human | Mab11 | PE-Cy7 | function/cytokine secretion | T helper | High |
| CD3 | Human | UCHT1 | Alexa Fluor 700 | lineage | T helper | High |
| IL-2 | Human | MQ1-17H12 | PerCP-Cy5.5 | function/cytokine secretion | T helper | Med |
