OMIP-007
Phenotypic analysis of human natural killer cells
Cytometry PART A, Volume 81A, Issue 6, 447-449 (2012)
Micheal A. Eller, Jeffrey R. Currier
Description: This panel was developed to characterize the phenotype of human natural killer (NK) cells from cryopreserved peripheral blood mononuclear cells (PBMC) isolated from ACD or EDTA anticoagulated whole blood or apheresis units. The application of this panel was to identify changes in NK cell subsets with regard to receptor expression, maturation, homing potential, and activation in the setting of primary HIV-1 natural infection. However, this panel may be applied to a wide variety of disease and normal conditions to characterize NK cells in humans. The performance of this panel was tested on fresh and frozen PBMC as well as using a whole blood lyse no wash procedure.
Species: Human
Tissue: Peripheral blood
Cell Subset: Nk, Nkt
Keyword: Cryopreserved pbmc, Hiv
Machine: LSR II
Panel details
| Marker | Species | Clone | Fluorophore | Purpose | Co-expressions groups | Antigen Density |
|---|---|---|---|---|---|---|
| CD16 | Human | 3G8 | Pacific Blue | NK subset | NK | Medium |
| Integrin alpha 4 beta 7 | Human | ACT-1 | Qdot 655 | Homing | NK, NKT | Low |
| CD62L | Human | DREG56 | Qdot 605 | Homing | NKT | Medium |
| CD57 | Human | hCD57 | APC | Differentiation | NK, NKT | High |
| Dead cells | Human | Live/Dead Fix Aqua | Viability | |||
| CD4 | Human | SFCI12T4D11 | ECD | Exclusion | High | |
| CD14 | Human | Tuk4 | PE-Cy5 | Exclusion | High | |
| CD19 | Human | SJ25-C1 | PE-Cy5 | Exclusion | Low | |
| CD56 | Human | NCAM16.2 | PE-Cy7 | NK subset | NK, NKT | Low |
| CD158 | Human | HP-MA4 | PerCP-Cy5.5 | KIR receptor | NK, NKT | Medium |
| CD158b1/b2,j | Human | DX27 | PE | KIR receptor | NK, NKT | Low |
| HLA DR | Human | G46-6 | FITC | Activation | NK | High |
| CD8a | Human | SK1 | APC-H7 | NK subset | NKT | High |
| CD3 | Human | S4.1 | PE-Texas Red | Exclusion | NK, NKT | High |
| CD158e | Human | DX9 | Alexa Fluor 700 | KIR receptor | NK, NKT | Low |
