OMIP-066
Identification of Novel Subpopulations of Human Group 2 Innate Lymphoid Cells in Peripheral Blood
Cytometry PART A, Volume 97, Issue 10, 1028-1031 (2020)
Yoichiro Ohne
Description: This 14‐color flow cytometry panel was designed to identify newly described subpopulations within human group 2 innate lymphoid cells (ILC2s) and other ILC subsets. This panel also allowed to identify recently reported subpopulations of peripheral blood CRTH2− c‐Kit+ ILCs. We validated this panel mostly in human peripheral blood but also confirmed that the same panel and gating strategy works well in human tonsillar cells. The panel contains a few markers indicating the activation status of ILCs. In addition, phycoerythrin (PE) channel is available for the markers of interest in each study. In the validation studies described here, PE channel was used to test the expression of some markers. These features make this panel applicable for immunophenotyping of ILCs in various disease states.
Species: Human
Tissue: Peripheral blood, Tonsil
Cell Subset: Ilc, Ilc1, Ilc2, Ilc3a, Ilc3b, Nk
Keyword: Activation
Machine: BD FACSymphony A5
Panel details
| Marker | Species | Clone | Fluorophore | Purpose | Co-expressions groups | Antigen Density |
|---|---|---|---|---|---|---|
| CD117 | Human | 104D2 | PE-Cy7 | Subpopulation | ILC3 | Medium |
| CD3 | Human | SK7 | Brilliant Violet 510 | Lineage/Dump | High | |
| CCR6 | Human | G034E3 | Brilliant Violet 421 | Lineage/Dump | ILC2 | Medium |
| Dead cells | Human | Live/Dead Fix Blue | Viability | |||
| KLRG1 | Human | 13F12F2 | SuperBright 702 | Subpopulation | NK | Low |
| CD56 | Human | NCAM16.2 | APC-R700 | Subpopulation | NK | Low |
| CCR10 | Human | 1B5 | Brilliant Ultraviolet 395 | Subpopulation | ILC2 | Medium |
| CD45 | Human | HI30 | Brilliant Ultraviolet 805 | Lymphocyte | NK. ILC1, ILC2, ILC3 | High |
| CD4 | Human | RPA-T4 | Brilliant Violet 510 | Lineage/Dump | High | |
| CD14 | Human | M5E2 | Brilliant Violet 510 | Lineage/Dump | High | |
| CD34 | Human | 581 | Brilliant Violet 510 | Lineage/Dump | Medium | |
| CD123 | Human | 6H6 | Brilliant Violet 510 | Lineage/Dump | Medium | |
| CD336 | Human | p44-8 | Brilliant Violet 786 | ILC3 in tonsil | ILC3 | Low |
| CD127 | Human | REA614 | Vio Bright 515 | Total ILC | ILC1, ILC2, ILC3 | Medium |
| TSLP Receptor | Human | 1F11 | Brilliant Blue 700 | ILC2 activation | ILC2 | Low |
| NKp46 | Human | DX22 | PE-Dazzle 594 | Subpopulation | NK | Low |
| CD294 | Human | BM16 | Alexa Fluor 647 | ILC2 | ILC2 | Medium |
| CD94 | Human | 9E2 | PE-Dazzle 594 | NK | NK | Medium |
| CD19 | Human | HIB19 | Brilliant Violet 510 | Lineage/Dump | Low |
