Description: The purpose of the panel described here is to assess the immune cell composition and their functionality in the peripheral blood mononuclear cells (PBMCs) of dogs. Moreover, its “plug and play” composition allows for an in‐depth analysis of T‐cell responses in ex vivo assays. Initially, this panel has been designed for the analysis of cryopreserved PBMCs to allow batched analysis and to reduce interexperimental variation. Withers and colleagues published a comparable and—to our knowledge—currently the most extensive canine panel to date. While their study focused on the aging and activation status of T cells in dogs, our panel is designed to look at a broader range of cells with a higher number of markers. This allows a more in‐depth analysis of functional extracellular and intracellular markers. In addition, all antibodies in our proposed panel are directly labeled. In combination with suitable lymphocyte isolation protocols, this panel could potentially also be adapted to analyze tissue biopsies from various different organs.

Species: Dog

Tissue: Peripheral blood

Cell Subset: B cell, T cell, Ctl, T helper, Treg, Nk, Monocyte

Keyword: Immunoprofiling

Machine: BD LSR Fortessa