Description: The panel was developed and optimized to identify mouse bone marrow hematopoietic stem cells (HSCs) and five multipotent progenitors (MPPs) along with controllable green fluorescent protein (GFP) expression as well as CD45.1 and CD45.2. HSCs and MPPs can be identified by analyzing the cell surface proteins Sca1, cKit, CD150, CD48, CD34, and CD135. The common myeloid progenitor (CMP), the granulocyte macrophage progenitor (GMP), the megakaryocyte erythrocyte progenitor (MEP), and the common lymphoid progenitor (CLP) are defined by specific expression patterns of Sca1 and cKit plus CD16/32 and CD34, CD135, and CD127. Additionally, the membrane integrity is determined with a life/dead discriminator to differentiate living from dead cells. The staining is performed in freshly isolated living cells without fixation obtained from transgenic mice (SCL‐tTA H2B‐GFP or ISRE‐EGFP) expressing the GFP and the genetic alleles CD45.1 or CD45.2. The analysis of the status of these markers is included in the reported 16 parameter staining panel.

Species: Mouse

Tissue: Bone marrow

Cell Subset: Progenitors/ hsc, Hsc, Gmp/gmdp, Cmp, Mpp, Mep, Clp

Keyword: Hematopoeisis

Machine: BD FACSAria Fusion