OMIPs Directory

OMIP-055

Characterization of Human Innate Lymphoid Cells from Neonatal and Peripheral Blood

Cytometry PART A, Volume 95, Issue 4, 427-430 (2019)
Sabrina Bianca Bennstein, Angela Riccarda Manser, Sandra Weinhold, Nadine Scherenschlich, Markus Uhrberg

Description: This OMIP panel was designed to characterize the different subsets of human innate lymphoid cells (ILCs) including ILC1, ILC2, and ILC3 within healthy donors' cord blood (CB) in comparison to peripheral blood (PB). As ILCs represent a rare cell population in blood (0.1–0.5% of lymphocytes), the protocol was designed to rigorously exclude the contaminating cells with a complex lineage‐depleting antibody mixture (13 different antibodies) enabling analysis of highly pure ILC subsets by 8‐color staining.

Species: Human

Tissue: Cord blood, Peripheral blood

Cell Subset: Ilc, Ilc1, Ilc2, Ilc3a, Ilc3b

Machine: Beckman Coulter CytoFLEX

Panel details

Marker Species Clone Fluorophore Purpose Co-expressions groups Antigen Density
Dead cells Human Live/Dead Fix Green Viability
CD1a Human HI149 FITC exclude DC Low
CD14 Human hCD14 FITC exclude monocyte High
CD19 Human HIB19 FITC exclude B cell Low
CD3 Human UCHT1 FITC exclude T, NKT High
TCR gamma/delta Human B1 FITC exclude CD3-TCRgd Med
CD123 Human 6H6 FITC exclude DC Med
CD303 Human 201A FITC exclude pDC (plasmacytoid DC) High
Fc epsilon R1 alpha Human AER-37 (CRA-1) FITC exclude mast cells, basophils High
CD235a Human HI264 FITC exclude erythrocytes and erythroid precursor High
CD66b Human G10F5 FITC exclude granulocyte High
CD34 Human 581 FITC exclude HSPC Med
CD94 Human DX22 PE-Cy7 NK NK Med
CD117 Human 104D2 Brilliant Violet 421 ILC3 cell surface receptor ILC Med
CD294 Human BM16 PE-Dazzle 594 ILC2 cell surface receptor ILC Med
CD45 Human HI30 APC-Cy7 leucocyte ILC, NK High
CD161 Human HP-3G10 Alexa Fluor 700 ILC inclusion marker (especially for tissue) ILC Low
TCR alpha/beta Human IP26 FITC exclude TCRab+ Med
CD20 Human 2H7 FITC exclude B cell Med
CD127 Human R34.34 PE-Cy5 ILC inclusion marker ILC Med
CD56 Human HCD56 Brilliant Violet 650 NK, ILCs ILC, NK Low