An 18‐Color Panel for Measuring Th1, Th2, Th17, and Tfh Responses in Rhesus Macaques

Cytometry PART A, Volume 95, Issue 3, 261-263 (2019)
Mitzi M. Donaldson, Shing‐Fen Kao, Kathryn E. Foulds

Description: This 18‐color panel was designed to measure vaccine‐induced T cell responses in rhesus macaques by intracellular cytokine staining (ICS). It detects seven cytokines, IFN‐γ, IL‐2, IL‐4, IL‐5, IL‐13, IL‐17, and IL‐21, two memory markers, CD45RA and CCR7, and four follicular helper T cell (Tfh) markers, CXCR3, CXCR5, ICOS, and PD‐1. While this panel was optimized for use on cryopreserved rhesus macaque peripheral blood mononuclear cells (PBMC), it can also be used on rhesus macaque tissue samples and human PBMC and tissue samples.

Species: Human, Rhesus macaque

Tissue: Peripheral blood

Cell Subset: T cell, Ctl, T helper, T memory, Th1, Th17, Th2, Treg, Nk, Tfh

Keyword: Cryopreserved pbmc, Cryopreserved tissue, Immune checkpoint

Machine: BD LSR2

Panel details

Marker Species Clone Fluorophore Purpose Co-expressions groups Antigen Density
IL-5 Rhesus macaque JES1-39D10 Brilliant Blue 515 cytokine T cell Low
Dead cells Rhesus macaque Live/Dead Fix Aqua Viability
CD3 Rhesus macaque SP34-2 APC-Cy7 T cell T cell, Tfh High
CD4 Rhesus macaque S3.5 PE-Cy5.5 T cell T cell, Tfh High
CD69 Rhesus macaque TP1.55.3 ECD background reduction T cell Med
IFN-gamma Rhesus macaque B27 Alexa Fluor 700 cytokine T cell, Tfh High
IL-17 Rhesus macaque BL168 Brilliant Violet 605 cytokine T cell, Tfh Low
IL-21 Rhesus macaque 3A3-N2.1 Alexa Fluor 647 cytokine T cell, Tfh Low
CD45RA Rhesus macaque 5H9 PE-Cy5 memory marker T cell, T memory Med
CD185 Rhesus macaque MU5UBEE PE Tfh T cell, Tfh Low
PD-1 Rhesus macaque EH12.2H7 Brilliant Violet 785 Tfh T cell, Tfh Low
CD278 Rhesus macaque C398.4A PE-Cy7 Tfh T cell, Tfh Low
CD8a Rhesus macaque RPA-T8 Brilliant Violet 570 T cell T cell High
CD183 Rhesus macaque 1C6/CXCR3 Brilliant Violet 711 Tfh T cell, Tfh Med
CCR7 Rhesus macaque G043H7 Brilliant Violet 650 memory marker T cell, T memory Low
IL-4 Rhesus macaque MP4-25D2 Brilliant Blue 700 cytokine T cell, Tfh Low
IL-13 Rhesus macaque JES10-5A2 Brilliant Violet 421 cytokine T helper, Tfh Low
IL-2 Rhesus macaque MQ1-17H12 Brilliant Violet 750 cytokine T cell, Tfh Med