OMIP-052
An 18‐Color Panel for Measuring Th1, Th2, Th17, and Tfh Responses in Rhesus Macaques
Cytometry PART A, Volume 95, Issue 3, 261-263 (2019)
Mitzi M. Donaldson, Shing‐Fen Kao, Kathryn E. Foulds
Description: This 18‐color panel was designed to measure vaccine‐induced T cell responses in rhesus macaques by intracellular cytokine staining (ICS). It detects seven cytokines, IFN‐γ, IL‐2, IL‐4, IL‐5, IL‐13, IL‐17, and IL‐21, two memory markers, CD45RA and CCR7, and four follicular helper T cell (Tfh) markers, CXCR3, CXCR5, ICOS, and PD‐1. While this panel was optimized for use on cryopreserved rhesus macaque peripheral blood mononuclear cells (PBMC), it can also be used on rhesus macaque tissue samples and human PBMC and tissue samples.
Species: Human, Rhesus macaque
Tissue: Peripheral blood
Cell Subset: T cell, Ctl, T helper, T memory, Th1, Th17, Th2, Treg, Nk, Tfh
Keyword: Cryopreserved pbmc, Cryopreserved tissue, Immune checkpoint
Machine: BD LSR2
Panel details
Marker | Species | Clone | Fluorophore | Purpose | Co-expressions groups | Antigen Density |
---|---|---|---|---|---|---|
IL-5 | Rhesus macaque | JES1-39D10 | Brilliant Blue 515 | cytokine | T cell | Low |
Dead cells | Rhesus macaque | Live/Dead Fix Aqua | Viability | |||
CD3 | Rhesus macaque | SP34-2 | APC-Cy7 | T cell | T cell, Tfh | High |
CD4 | Rhesus macaque | S3.5 | PE-Cy5.5 | T cell | T cell, Tfh | High |
CD69 | Rhesus macaque | TP1.55.3 | ECD | background reduction | T cell | Med |
IFN-gamma | Rhesus macaque | B27 | Alexa Fluor 700 | cytokine | T cell, Tfh | High |
IL-17 | Rhesus macaque | BL168 | Brilliant Violet 605 | cytokine | T cell, Tfh | Low |
IL-21 | Rhesus macaque | 3A3-N2.1 | Alexa Fluor 647 | cytokine | T cell, Tfh | Low |
CD45RA | Rhesus macaque | 5H9 | PE-Cy5 | memory marker | T cell, T memory | Med |
CD185 | Rhesus macaque | MU5UBEE | PE | Tfh | T cell, Tfh | Low |
PD-1 | Rhesus macaque | EH12.2H7 | Brilliant Violet 785 | Tfh | T cell, Tfh | Low |
CD278 | Rhesus macaque | C398.4A | PE-Cy7 | Tfh | T cell, Tfh | Low |
CD8a | Rhesus macaque | RPA-T8 | Brilliant Violet 570 | T cell | T cell | High |
CD183 | Rhesus macaque | 1C6/CXCR3 | Brilliant Violet 711 | Tfh | T cell, Tfh | Med |
CCR7 | Rhesus macaque | G043H7 | Brilliant Violet 650 | memory marker | T cell, T memory | Low |
IL-4 | Rhesus macaque | MP4-25D2 | Brilliant Blue 700 | cytokine | T cell, Tfh | Low |
IL-13 | Rhesus macaque | JES10-5A2 | Brilliant Violet 421 | cytokine | T helper, Tfh | Low |
IL-2 | Rhesus macaque | MQ1-17H12 | Brilliant Violet 750 | cytokine | T cell, Tfh | Med |