OMIP-051
28‐color flow cytometry panel to characterize B cells and myeloid cells
Cytometry PART A, Volume 95, Issue 2, 105-155 (2019)
Thomas Liechti, Mario Roederer
Description: This 28‐color flow cytometry panel focuses on B cells, dendritic cells, and monocytes and was optimized for cryopreserved peripheral blood mononuclear cells (PBMC). In addition to markers enabling the analysis of monocytes (CD14) and definition of subsets within B cells (CD10, CD19, CD20, CD21, CD27, IgD, IgM) and dendritic cells (CD1c, CD11c, CD123, CD141, HLA‐DR), we included functional markers such as chemokine receptors (CXCR3, CXCR5), surface immunoglobulins (IgA, IgD, IgG, IgM), Fc receptors (CD16, CD23, CD32, CD64), inhibitory molecules (CD73, CD85j), the co‐stimulatory molecule CD40, and cytokine receptors (IL‐21R, BAFF‐R, TACI), which enables in‐depth characterization of B cells, dendritic cells, and monocytes.
Species: Human
Tissue: Peripheral blood
Cell Subset: B cell, B memory, Dc, Mdc/cdc, Pdc, Monocyte
Keyword: Activation, Cell migration
Machine: BD FACSymphony A5
Panel details
| Marker | Species | Clone | Fluorophore | Purpose | Co-expressions groups | Antigen Density |
|---|---|---|---|---|---|---|
| CD141 | Human | 1A4 | Brilliant Blue 630-P2 | DC lineage marker | DC, Monocyte | Low |
| CD123 | Human | 7G3 | Brilliant Blue 660-P2 | DC lineage marker | DC | Med |
| CD21 | Human | B-ly4 | Brilliant Ultraviolet 496 | Differentiation B cells | B cell | Low |
| CD267 | Human | 1A1-K21-M22 | Brilliant Ultraviolet 563 | Cytokine receptor | B cell | Low |
| CD185 | Human | RF8B2 | Brilliant Violet 750 | Trafficking | B cell | Med |
| Dead cells | Human | Live/Dead Fix Blue | viability' | |||
| CD73 | Human | AD2 | Brilliant Blue 515 | Inhibitory | B cell, DC | Med |
| CD16 | Human | 3G8 | Brilliant Blue 700 | Fc receptor | Monocyte, DC | Med |
| CD40 | Human | 5C3 | PE-Dazzle 594 | Co-stimulatory | B cell, DC | Med |
| CD85j | Human | GHI/75 | PE-Cy5 | inhibitory | B cell | Low |
| CD11c | Human | Bu15 | PE-Cy5.5 | DC lineage marker | mDC | Med |
| CD183 | Human | G025H7 | PE-Cy7 | Trafficking | B cell | Low |
| CD27 | Human | M-T271 | APC-R700 | Differentiation B cells | B cell | Med |
| CD19 | Human | SJ25C1 | APC-H7 | Lineage B cell | B cell | Low |
| CD20 | Human | 2H7 | Brilliant Ultraviolet 805 | Lineage B cell | B cell | Med |
| IL-21 R | Human | 2G1-K12 | Brilliant Violet 421 | Cytokine receptor | B cell | Low |
| IgM | Human | MHM-88 | Brilliant Violet 570 | Differentiation B cells | B cell | Med |
| CD268 | Human | 11C1 | Brilliant Violet 605 | Cytokine receptor | B cell | Med |
| CD10 | Human | HI10a | Brilliant Violet 650 | Differentiation B cells | B cell | Low |
| CD23 | Human | M-L233 | Brilliant Violet 711 | Differentiation B cells | B cell, DC, Monocyte | Med |
| CD64 | Human | 10.1 | Brilliant Violet 786 | Fc receptor | Monocyte, DC | Med |
| CD1c | Human | F10/21A3 | Brilliant Ultraviolet 395 | DC lineage marker | B cell, mDC | Med |
| HLA DR | Human | G46-6 | Brilliant Ultraviolet 661 | DC lineage marker | mDC, B cell | Med |
| CD14 | Human | MphiP9 (also known as MphiP-9) | Brilliant Violet 510 | Lineage monocyte | Monocyte | High |
| IgA | Human | polyclonal | APC | Differentiation B cells | B cell | High |
| IgG | Human | Brilliant Ultraviolet 737 | Differentiation B cells | B cell | High | |
| IgD | Human | IA6-2 | Brilliant Blue 790-P | Differentiation B cells | B cell | High |
| CD32 | Human | FUN-2 | PE | Fc receptor | B cell, mDC, Monocyte | Med |
