OMIPs Directory

OMIP-046

Characterization of invariant T cell subset activation in humans

Cytometry PART A, Volume 93, Issue 5, 499-503 (2018)
Kerri G. Lal, Edwin Leeansyah, Johan K. Sandberg, Michael A. Eller

Description: This panel was developed to characterize the two most commonly studied invariant T cell subsets: mucosal‐associated invariant T (MAIT) cells and invariant natural killer T (iNKT) cells in humans and measure their expression of exhaustion and activation markers. The panel was optimized and designed for application in cryopreserved peripheral blood mononuclear cells (PBMC). Designed to be used to measure frequency and phenotype of invariant T cell subtypes in acute HIV‐1 infection, optimization was performed on chronically HIV‐1 infected and uninfected donors and can be applied to a variety of human disease cohorts.

Species: Human

Tissue: Peripheral blood

Cell Subset: Mait, Nk, Nkt

Keyword: Cryopreserved pbmc, Hiv, Immune checkpoint

Machine: BD LSR2 SORP

Panel details

Marker Species Clone Fluorophore Purpose Co-expressions groups Antigen Density
TIGIT Human MBSA43 PE-Cy7 Exhaustion T cell, NK, NKT Med
CD8a Human SK1 PerCP-Cy5.5 T cell lineage T cell High
Dead cells Human Live/Dead Fix Aqua Viability
TCR V alpha 24 Human C15 FITC iNKT NKT Med
CD57 Human hCD57 Pacific Blue Activation NK High
CD161 Human HP-3G10 Brilliant Violet 605 MAIT NK, MAIT Low
PD-1 Human EH12.1 Brilliant Violet 786 Exhaustion MAIT, T cell Low
TCR V alpha 7.2 Human 3C10 APC MAIT MAIT Med
CD14 Human M5E2 Alexa Fluor 700 Exclusion High
CD4 Human SK3 APC-H7 T cell lineage T cell High
TCR V beta 11 Human C21 PE iNKT NKT Med
CD3 Human S4.1 PE-Texas Red T cell lineage T cell High
HLA DR Human L243 Brilliant Violet 650 Activation T cell, MAIT High
CD19 Human HIB19 Alexa Fluor 700 Exclusion Low
CD38 Human HIT2 Brilliant Violet 711 Activation T cell Low