In-depth characterization of human T regulatory cells

Cytometry PART A, Volume 81A, Issue 1, 15-16 (2012)
Angelique Biancotto, Pradeep K. Dagur, J. Chris Fuchs, Marc Langweiler, J. Philip McCoy Jr

Description: The present panel was constructed for the in-depth characterization of human T regulatory cells in both health and disease. The panel works well in both fresh and cryopreserved PBMCs. The panel has also been tested on ACK-lysed peripheral blood. No other types of tissues have been tested.

Species: Human

Tissue: Peripheral blood

Cell Subset: T cell, T memory, Treg

Keyword: Cryopreserved pbmc

Machine: LSR II

Panel details

Marker Species Clone Fluorophore Purpose Co-expressions groups Antigen Density
Dead cells Human Live/Dead Fix Aqua Viability
CD3 Human SK7 APC-Cy7 T cell T memory, T reg High
CD4 Human RPA-T4 V450 T helper subset T memory, T reg High
CD25 Human M-A251 PE-Cy7 Treg characterization T reg Low
CD27 Human CLB-27/1 Qdot 655 Treg characterization T memory Medium
CD39 Human A1 Alexa Fluor 488 Treg supression T reg, T memory Low
CD103 Human LF61 PE-Cy5 naïve T cell T reg Low
CD127 Human hIL-7R-M21 Alexa Fluor 647 Treg characterization T reg Low
FOXP3 Human PCH101 PE Treg marker T reg Low
CD45RA Human 2H4LDH11LDB9 ECD naïve T cell T memory Medium
CD45 Human HI30 Qdot 800 leukocyte T reg, T memory High
HLA DR Human Tu36 PE-Cy5.5 Treg characterization T memory High
CCR7 Human 150503 Alexa Fluor 700 Treg characterization T memory Low
CD8a Human 3B5 Qdot 605 T suppressor subset T memory High
CD38 Human HIT2 PerCP-Cy5.5 Treg characterization T memory High