OMIP-004
In-depth characterization of human T regulatory cells
Cytometry PART A, Volume 81A, Issue 1, 15-16 (2012)
Angelique Biancotto, Pradeep K. Dagur, J. Chris Fuchs, Marc Langweiler, J. Philip McCoy Jr
Description: The present panel was constructed for the in-depth characterization of human T regulatory cells in both health and disease. The panel works well in both fresh and cryopreserved PBMCs. The panel has also been tested on ACK-lysed peripheral blood. No other types of tissues have been tested.
Species: Human
Tissue: Peripheral blood
Cell Subset: T cell, T memory, Treg
Keyword: Cryopreserved pbmc
Machine: LSR II
Panel details
| Marker | Species | Clone | Fluorophore | Purpose | Co-expressions groups | Antigen Density |
|---|---|---|---|---|---|---|
| Dead cells | Human | Live/Dead Fix Aqua | Viability | |||
| CD3 | Human | SK7 | APC-Cy7 | T cell | T memory, T reg | High |
| CD4 | Human | RPA-T4 | V450 | T helper subset | T memory, T reg | High |
| CD25 | Human | M-A251 | PE-Cy7 | Treg characterization | T reg | Low |
| CD27 | Human | CLB-27/1 | Qdot 655 | Treg characterization | T memory | Medium |
| CD39 | Human | A1 | Alexa Fluor 488 | Treg supression | T reg, T memory | Low |
| CD103 | Human | LF61 | PE-Cy5 | naïve T cell | T reg | Low |
| FOXP3 | Human | PCH101 | PE | Treg marker | T reg | Low |
| CD45RA | Human | 2H4LDH11LDB9 | ECD | naïve T cell | T memory | Medium |
| CD45 | Human | HI30 | Qdot 800 | leukocyte | T reg, T memory | High |
| HLA DR | Human | Tu36 | PE-Cy5.5 | Treg characterization | T memory | High |
| CD197 (CCR7) | Human | 150503 | Alexa Fluor 700 | Treg characterization | T memory | Low |
| CD8a | Human | 3B5 | Qdot 605 | T suppressor subset | T memory | High |
| CD38 | Human | HIT2 | PerCP-Cy5.5 | Treg characterization | T memory | High |
| CD127 | Human | HIL-7R-M21 | Alexa Fluor 647 | Treg characterization | T reg | Low |
