OMIP-039
Detection and analysis of human adaptive NKG2C+ natural killer cells
Cytometry PART A, Volume 91, Issue 10, 997–1000 (2017)
Quirin Hammer, Chiara Romagnani
Description: The present panel was optimized for the detection of adaptive natural killer (NK)‐cell populations in healthy human donors and offers versatility to investigate their biology including receptor usage, activation requirements, or signaling adaptor and transcription factor expression. It was established on cryopreserved PBMC and yields similar results with freshly isolated PBMC. Additional tissue samples have not been tested.
Species: Human
Tissue: Peripheral blood
Cell Subset: Nk
Keyword: Activation, Cryopreserved pbmc
Machine: BD LSR Fortessa
Panel details
| Marker | Species | Clone | Fluorophore | Purpose | Co-expressions groups | Antigen Density |
|---|---|---|---|---|---|---|
| CD159a (NKG2A) | Human | REA110 | PE-Vio770 | Conventional NK | Conventional NK | Low |
| Dead cells | Human | Zombie Aqua | viability | |||
| CD14 | Human | M5E2 | Brilliant Violet 510 | exclusion | High | |
| CD19 | Human | HIB19 | Brilliant Violet 510 | exclusion | Low | |
| CD3 | Human | UCHT1 | PE-Cy5 | exclusion | High | |
| CD337 | Human | AF29-4D12 | eFluor 450 | Conventional NK | Conventional NK | Low |
| CD328 | Human | REA214 | APC-Vio770 | Conventional NK | Conventional NK | High |
| CD85j | Human | HP-F1 | APC | Adaptive NK | Adaptive NK | Low |
| IgM | Human | RMM-1 | Brilliant Violet 605 | secondary reagent for CD57 | Adaptive NK | |
| CD7 | Human | M-T701 | Brilliant Violet 786 | Conventional NK | Conventional NK | Med |
| CD57 | Human | TBO1 | Adaptive NK | Adaptive NK | High | |
| CD2 | Human | RPA-2.10 | PerCP-Cy5.5 | Adaptive NK | Adaptive NK | Med |
| CD56 | Human | HCD56 | PE-Dazzle 594 | NK cells | Conventional NK, Adaptive NK | Low |
| CD159c | Human | REA205 | PE | Adaptive NK | Adaptive NK | Low |
