OMIP-038
Innate immune assessment with a 14 color flow cytometry panel
Cytometry PART A, Volume 91, Issue 10, 966–968 (2017)
Kinga K. Smolen, Bing Cai, Tobias R. Kollmann
Description: The purpose of the panel presented here is to assess the innate immune response in whole blood after Toll‐like receptor (TLR) ligand or cytokine stimulation, at the single‐cell level. In addition to the identification of many of the innate and innate‐adaptive interface immune cell subsets, this panel also enables the detection of key intracellular cytokines involved in the immune response.
Species: Human
Tissue: Peripheral blood
Cell Subset: Neutrophil, T cell, Mdc/cdc, Pdc, Nk, Monocyte
Keyword: Activation, Toll-like receptor (tlr)
Machine: BD LSR2
Panel details
| Marker | Species | Clone | Fluorophore | Purpose | Co-expressions groups | Antigen Density |
|---|---|---|---|---|---|---|
| IL-12 | Human | C8.6 | eFluor 450 | Function | Monocyte | Med |
| IL-6 | Human | MQ2-13A5 | PerCP-eFluor 710 | Function | Monocyte | Low |
| TCR gamma/delta | Human | B1.1 | FITC | γδ T cell | TCRgd | Low |
| CD123 | Human | 6H6 | PE-Cy7 | pDC (plasmacytoid DC) | pDC | Med |
| Dead cells | Human | eFluor 780 Fix Viability | Live/Dead | |||
| CD66 | Human | ASL-32 | Neutrophil | Neutrophil | High | |
| CD3 | Human | UCHT1 | PE-CF594 | T lineage | T cell, TCRgd | High |
| CD14 | Human | M5E2 | V500 | Monocytes | Monocyte, mDC | High |
| IFN alpha | Human | 7N4-1 | PE | Function | pDC | Med |
| TNF | Human | Mab11 | Alexa Fluor 700 | Function | Monocyte, T cell | Med |
| Streptavidin | Human | Brilliant Violet 786 | secondary reagent to CD66 | Neutrophil | ||
| CD16 | Human | 3G8 | Brilliant Violet 650 | NK, NKT, monocyte | Neutrophil, NK, Monocyte | Med |
| HLA DR | Human | LN3 | eFluor 605NC | B cell, monocyte | mDC, pDC, Monocyte | High |
| CD11c | Human | S-HCL-3 | APC | cDC | mDC | Med |
| IFN-gamma | Human | 4S.B3 | Brilliant Violet 711 | Function | T cell | High |
