OMIPs Directory

OMIP-037

16-color panel to measure inhibitory receptor signatures from multiple human immune cell subsets

Cytometry PART A, Volume 91, 175–179 (2016)
Anna C. Belkina, Jennifer E. Snyder-Cappione

Description: The panel was developed to determine the combinational inhibitory receptor expression (“IR signatures”) of CD4+ T cells, CD8+ T cells, Natural Killer (NK) cells, invariant Natural Killer T (iNKT) cells, and gamma delta (γδ) T cells from individual human samples. The inhibitory receptors measured are PD-1, TIM-3, CD160, LAG-3, and TIGIT. The activation marker CD137 (4-1BB) is also included in the panel, as is IL-7Rα (CD127). This panel works well with cryopreserved PBMC from healthy and HIV-infected individuals well as fresh tumor specimens. For optimum performance of this panel with digested tumor specimens, the pan-lymphocyte marker CD45 is included (swapped for LAG-3). Other tissues/sample types have not yet been evaluated. Also, a modification of this panel has been optimized that includes CD45RO and CD25 in place of CD160 and CD137.

Species: Human

Tissue: Peripheral blood, Tumor

Cell Subset: T cell, T memory, Treg, Tcrgd, Nkt

Keyword: Hiv, Immune checkpoint, Tetramer

Machine: BD FACSAria II SORP

Panel details

Marker Species Clone Fluorophore Purpose Co-expressions groups Antigen Density
CD160 Human BY55 Alexa Fluor 488 Exhaustion T cell, TCRgd, NK, NKT Low
CD16 Human 3G8 FITC NK cell lineage, subsets NK Med
CD1d-PBSS7 tetramer Human APC iNKT lineage NKT Med
Dead cells Human Zombie NIR Viability
TCR gamma/delta Human B1 Brilliant Ultraviolet 395 TCRγδ TCRgd Low
CD3 Human OKT3 Brilliant Violet 510 T cell lineage T cell, TCRgd, Treg High
CD8a Human SK1 Brilliant Ultraviolet 805 Lineage T cell, T memory High
CD56 Human NCAM16.2 Brilliant Violet 786 NK lineage/subsets NK, NKT Low
LAG-3 Human 3DS223H PE-eFluor 610 Exhaustion T cell, NK, Treg, TCRgd Low
TIGIT Human MBSA43 PerCP-eFluor 710 Exhaustion T cell, NK, Treg Med
TIM-3 Human F38-2E2 PE-Cy7 Exhaustion T cell, TCRgd Low
CD4 Human RPA-T4 Alexa Fluor 700 Th lineage T cell, Treg High
CD14 Human hCD14 APC-Cy7 monocyte exclusion High
CD19 Human HIB19 APC-Cy7 B cell exclusion Low
CD45 Human HI30 PE-Dazzle 594 hematopoitic cell ineage T cell, TCRgd, NK, NKT, Treg High
CD137 Human 4B4-1 Brilliant Violet 650 Acivation T cell, TCRgd Med
CD45RO Human UCHL1 Brilliant Violet 650 naïve/memory T cell, T memory, TCRgd Med
CD25 Human BC96 Brilliant Violet 605 Treg, T cell activation T cell, Treg Low
PD-1 Human EH12.2H7 Brilliant Violet 421 Exhaustion T cell, TCRgd Low
CD16 Human 3G8 Brilliant Violet 605 NK lineage/subsets NK Med
CD127 Human hIL-7R-M21 Brilliant Ultraviolet 737 Exhaustion, Treg T cell, T memory, Treg, TCRgd Med
TCR V alpha 24 Human C15 PE iNKT lineage NKT Med