OMIPs Directory

OMIP-035

Functional analysis of natural killer cell subsets in macaques

Cytometry PART A, Volume 89, Issue 9, 799–802 (2016)
Kim L. Weisgrau, Moritz Ries, Nicholas Pomplun, David T. Evans, Eva G. Rakasz

Description: This panel was developed to measure the functional capability of natural killer (NK) cell subsets in rhesus macaques (Macaca mulatta). It includes markers to determine the frequency of cytokine secreting and cytotoxic NK cell subpopulations in peripheral blood mononuclear cell (PBMC) samples stimulated in vitro with human 721.221 cells. NK cell subsets were defined by the expression of killer cell immunoglobulin-like receptors (KIRs) Mamu-KIR3DL01 and Mamu-KIR3DL05, and differentiation antigens CD16 and CD56. The panel can be used to assess the functional capability of NK cells in a range of normal and pathologic conditions of captive bred rhesus macaques of Indian origin.

Species: Rhesus macaque

Tissue: Peripheral blood

Cell Subset: Nk

Keyword: Activation, Tetramer

Machine: SORP BD LSR 2

Panel details

Marker Species Clone Fluorophore Purpose Co-expressions groups Antigen Density
Dead cells Macaca mulatta Live/Dead Fix Near IR (775) viability
CD158 Macaca mulatta NKVFS1 PE NK subset NK Med
KIR3dl05 Macaca mulatta GY9 tetramer APC NK subset NK Low
CD3 Macaca mulatta SP34-2 PE-CF594 Exclusion High
CD16 Macaca mulatta 3G8 Pacific Blue NK subset NK Med
CD20 Macaca mulatta 2H7 PE-CF594 Exclusion Med
CD56 Macaca mulatta B159 PerCP-Cy5.5 NK subset NK Low
CD107a Macaca mulatta H4A3 Brilliant Violet 605 NK cell function NK Low
Granzyme B Macaca mulatta GB11 Brilliant Violet 510 NK cell function NK Med
IFN-gamma Macaca mulatta 4S.B3 FITC NK cell function NK High
CD8a Macaca mulatta RPA-T8 Brilliant Violet 711 NK subset NK High
CD159a (NKG2A) Macaca mulatta Z199 PE-Cy7 NK subset NK Med
TNF Macaca mulatta Mab11 Alexa Fluor 700 NK cell function NK Med
CD45 Macaca mulatta D058-1283 Brilliant Violet 786 hematopoitic cell lineage NK High