OMIPs Directory

OMIP-030

Characterization of human T cell subsets via surface markers

Cytometry PART A, Volume 87, Issue 12, 1067–1069 (2015)
Gerhard Wingender, Mitchell Kronenberg

Description: The present panel was optimized to quantify the relative frequency of the majority of the major T cell subsets currently described within human peripheral blood mononuclear cells (PBMCs) via the use of surface markers. This includes all CD4+ T subsets that received a T helper—nomenclature to date and Tregs. Furthermore, a surrogate staining strategy for the identification of mNKT/MAIT cells without the need of Vα7.2 is proposed. The panel has been validated for fresh and cryopreserved PBMCs. Other tissue types have not been tested.

Species: Human

Tissue: Peripheral blood

Cell Subset: T cell, T helper, Ctl, Treg, Mait, Nkt

Keyword: Cryopreserved pbmc

Machine: BD LSR Fortessa

Panel details

Marker Species Clone Fluorophore Purpose Co-expressions groups Antigen Density
CD25 Human 2A3 Brilliant Violet 711 Regulatory T cells T reg, T cell Low
CCR6 Human G034E3 Brilliant Violet 785 Subsets T helper, T cell Med
CD161 Human HP-3G10 PerCP-Cy5.5 mNKT/MAIT mNKT/MAIT Med
CD194 Human 1G1 PE Subsets T helper, T cell Med
CD197 (CCR7) Human 150503 PE-CF594 Naive/memory T memory, T cell Low
Ki-67 Human B56 Alexa Fluor 700 Proliferation T cell Low
CD183 (CXCR3) Human 1C6 Alexa Fluor 488 Subsets T helper, T cell Med
Streptavidin Human N/A PE-Cy7 T helper, T cell
CD185 Human RF8B2 Subsets T helper, T cell Low
CD127 Human eBioRDR5 APC-eFluor 780 Regulatory T cells Treg, T cell Low
CD38 Human HB7 eFluor 450 Activation T cell, Treg Low
CD20 Human 2H7 PE-Cy5.5 lineage Med
CD45RA Human HI100 Brilliant Violet 570 Naive/memory T memory, T cell Med
CD3 Human UCHT1 Qdot 605 lineage T cell, T memory, Treg, T helper High
CD8a Human RPT-T8 eFluor 650NC lineage T cell High
CCR10 Human 314305 APC Subsets T helper, T cell Low
CD4 Human SK3 Brilliant Violet 510 lineage Treg, T cell, T memory, T helper High