OMIP-029
Human NK-cell phenotypization
Cytometry PART A, Volume 87, Issue 11, 986-988 (2015)
Yolanda D. Mahnke, Margaret H. Beddall, Mario Roederer
Description: The present panel was optimized to enumerate natural killer (NK) cells within peripheral blood mononuclear cells (PBMC) and to determine their phenotype in terms of NK receptor and differentiation marker expression in healthy individuals. It works well with cryopreserved PBMC and we have observed similar results with fresh specimens. Other tissue types have not been tested.
Species: Human
Tissue: Peripheral blood
Cell Subset: Nk
Keyword: Cryopreserved pbmc
Machine: BD LSR2
Panel details
| Marker | Species | Clone | Fluorophore | Purpose | Co-expressions groups | Antigen Density |
|---|---|---|---|---|---|---|
| CD62L | Human | SK11 | Alexa Fluor 680 | Differentiation | NK | Medium |
| NKp46 | Human | Bab281 | PE-Cy5 | NK receptor | NK | Low |
| CD3 | Human | SK7 | APC-H7 | exclusion | High | |
| CD4 | Human | OKT4 | Brilliant Violet 605 | exclusion | High | |
| CD16 | Human | 3G8 | Brilliant Violet 421 | NK | NK | Med |
| CD56 | Human | HCD56 | Brilliant Violet 570 | NK | NK | Low |
| CD158 | Human | HP-MA4 | FITC | NK receptor | NK | Med |
| CD314 | Human | 1D11 | PE-Cy7 | NK receptor | NK | Low |
| CD337 | Human | p30-15 | Alexa Fluor 647 | NK receptor | NK | Low |
| CD2 | Human | S5.5 | PE-Cy5.5 | NK | NK | Med |
| CCR7 | Human | 150503 | PE-CF594 | Differentiation | NK | Low |
| CD158b/j | Human | DX27 | PE | NK receptor | NK | Low |
| Live/Dead Fix Aqua | Human | Live/Dead Fix Aqua | Viability/exclusion |
