OMIP-028
Activation panel for Rhesus macaque NK cell subsets
Cytometry PART A, Volume 87, Issue 10, 890-893 (2015)
Nicholas Pomplun, Kim L. Weisgrau, David T. Evans, Eva G. Rakasz
Description: This panel was developed to quantify natural killer (NK) cell subsets in Rhesus macaques (Macaca mulatta) during SIVmac239 infection induced pathogenesis. It includes markers to monitor changes in the activation/proliferation phenotype of up to 12 NK cell populations. The performance of the staining was tested on cryopreserved lymph node samples, and on fresh and cryopreserved peripheral blood mononuclear cells (PBMC) isolated from EDTA anti-coagulated blood. The panel can be used to characterize NK cells in a range of normal and pathologic conditions of this species and can be easily adapted to stain samples from various tissues.
Species: Rhesus macaque
Tissue: Lymph node, Peripheral blood
Cell Subset: Nk
Keyword: Activation, Cryopreserved pbmc, Cryopreserved tissue, Proliferation, Sivmac239
Machine: BD LSR2
Panel details
| Marker | Species | Clone | Fluorophore | Purpose | Co-expressions groups | Antigen Density |
|---|---|---|---|---|---|---|
| KIR3dl05 | Macaca mulatta | Brilliant Violet 421 | NK subset | NK, NK subset | Low | |
| NKp46 | Macaca mulatta | Bab281 | PE-Cy5 | NK differentiation | NK | Low |
| Live/Dead Fix Near IR (775) | Macaca mulatta | Live/Dead Fix Near IR (775) | viability/exclusion | |||
| CD3 | Macaca mulatta | SP34-2 | Alexa Fluor 700 | exclusion | High | |
| CD14 | Macaca mulatta | M5E2 | Alexa Fluor 700 | exclusion | High | |
| CD16 | Macaca mulatta | 3G8 | Brilliant Violet 711 | NK subset | NK, NK subset | Med |
| CD20 | Macaca mulatta | 2H7 | Alexa Fluor 700 | exclusion | Med | |
| CD45 | Macaca mulatta | D058-1283 | Brilliant Violet 786 | hematopoietic cell lineage | NK | High |
| CD56 | Macaca mulatta | B159 | FITC | NK subset | NK, NK subset | Low |
| CD69 | Macaca mulatta | TP1.55.3 | ECD | Activation | NK | Med |
| Ki-67 | Macaca mulatta | B56 | Alexa Fluor 647 | Proliferation | NK | Low |
| PD-1 | Macaca mulatta | EH12.2H7 | Brilliant Violet 605 | NK differentiation | NK | Low |
| CD8a | Macaca mulatta | SK1 | Brilliant Violet 510 | NK subset | NK, NK subset | High |
| CD159a (NKG2A) | Macaca mulatta | Z199 | PE-Cy7 | NK subset | NK, NK subset | Low |
| HLA DR | Macaca mulatta | L243 | Brilliant Violet 650 | Activation | NK | Med |
