OMIPs Directory

OMIP-024

Pan-leukocyte immunophenotypic characterization of PBMC subsets in human samples

Cytometry PART A, Volume 85, Issue 12, 995-998 (2014)
Gemma Moncunill, Hannah Han, Carlota Dobano, M. Juliana McElrath, Stephen C. De Rosa

Description: This phenotyping panel was developed to measure the relative frequencies of multiple leukocyte cell subsets in peripheral blood mononuclear cells (PBMC) from African infants and children, including the expression of immune activation and differentiation markers. It was optimized with the objective of obtaining the maximum information concerning the immune status and cell subsets that could influence the immune response to vaccines and infectious diseases using small volumes of pediatric samples. It was developed using cryopreserved PBMC from healthy HIV-uninfected and HIV-infected US adults, but it has also been tested with cryopreserved PBMC from US infants. Although we have not tested the panel on whole blood, it is likely that the panel could be used with whole blood following minimal optimization.

Species: Human

Tissue: Peripheral blood

Cell Subset: T cell, T helper, T memory, Ctl, Treg, Tcrgd, Nk, Monocyte

Keyword: Activation, Cryopreserved pbmc, Hiv, Infant, Vaccination

Machine: BD LSR2

Panel details

Marker Species Clone Fluorophore Purpose Co-expressions groups Antigen Density
CD3 Human UCHT1 ECD T cell lineage Treg, T cell, TCRgd High
CD8a Human SK1 PerCP-Cy5.5 T cell lineage T cell, TCRgd High
Dead cells Human Live/Dead Fix Aqua viability
CD19 Human SJ25C1 Brilliant Ultraviolet 737 B cell B cell Low
CD25 Human M-A251 Brilliant Violet 421 Treg Treg, T cell Low
CD56 Human HCD56 Brilliant Violet 605 NK, NKT-like cell NK Low
CD14 Human MfP9 Brilliant Violet 711 monocyte monocyte High
CD57 Human NK-1 FITC NK cells NK High
TCR V delta 2 Human B6 PE γδ T cell TCRgd Med
CD38 Human HIT2 PE-Cy5 Activation, plasmablasts T cell, B cell, Treg, monocyte Low
HLA DR Human L243 Brilliant Violet 570 Activation monocyte, T cell, B cell High
CD197 (CCR7) Human G043H7 Brilliant Violet 785 memory, differentiation T memory, T cell Low
TCR gamma/delta Human 11F2 PE-Cy7 γδ T cell TCRgd Low
CD159c Human 134591 Alexa Fluor 700 NK receptor NK High
CD16 Human 3G8 APC-Cy7 NK, monocyte NK, monocyte, TCRgd Med
CD127 Human A019D5 APC Treg, memory, differentiation T cell, Treg Low
CD4 Human SK3 Brilliant Ultraviolet 395 T cell Treg, T cell High
CD45RA Human HI100 Brilliant Violet 650 memory,differentiation T memory, T cell Med