OMIPs Directory

OMIP-022

Comprehensive assessment of antigen-specific human T-cell functionality and memory

Cytometry PART A, Volume 85, Issue 7, 576-579 (2014)
Andrew J. Graves, Marcelino G. Padilla, David A. Hokey

Description: This flow cytometry antibody panel was developed and optimized for the characterization of CD4+ and CD8+ T-cell memory and functional responses in adult and infant cryopreserved peripheral blood mononuclear cells (PBMC) stimulated with peptide pools to various antigens of interest. The panel has been used to evaluate Mycobacterium tuberculosis (TB) antigen-specific responses in clinical trial specimens, and is currently undergoing assay qualification.

Species: Human

Tissue: Peripheral blood

Cell Subset: T cell, T helper, T memory, Th1, Th17, Th2, Th22, Ctl, Treg

Keyword: Antigen-specific t cell response, Cryopreserved pbmc, Infant, Tuberculosis (tb)

Machine: BD LSR2

Panel details

Marker Species Clone Fluorophore Purpose Co-expressions groups Antigen Density
Dead cells Human Live/Dead Fix Aqua viability
CD14 Human M5E2 V500 exclusion High
CD19 Human HIB19 V500 exclusion Low
CD4 Human RPA-T4 APC-eFluor 780 T cell subset T cell, T helper, T memory High
CD45RO Human UCHL1 Brilliant Violet 785 Memory T cell, T memory High
IFN-gamma Human B27 V450 Th1 T cell, CTL, T helper High
IL-22 Human IL22JOP APC Th22 T cell, CTL, T helper Low
CD107a Human H4A3 Alexa Fluor 488 Degranulation T cell, CTL Low
CD8a Human Hit8a Alexa Fluor 700 T cell subset T cell, CTL, T memory High
CCR7 Human G043H7 Brilliant Violet 605 Memory T cell, T memory Low
TNF Human Mab11 PE-Cy7 T helper subsets T cell, CTL, T helper Med
CD40L Human TRAP1 PE-Cy5 Activation/B-Cell help T cell, T memory Med
IL-17A Human BL168 PerCP-Cy5.5 Th17 T cell, CTL, T helper Low
CD3 Human UCHT1 ECD T cell T cell, CTL, T memory, T helper High
IL-2 Human MQ1-17H12 PE T helper subsets T cell, T helper, CTL Med